Filter
–
- Shop-all-products
Service hotline
Telephone support and professional advice under:
+49(0)731-3608-123
Or via our contact form.
In Vitro RNA Synthesis – Enzymes, Kits & Reagents
Everything You Need for In Vitro RNA Transcription – Kits, Enzymes, and More
In vitro RNA transcription is a key method in molecular biology for synthesizing RNA from DNA templates using dedicated RNA polymerases such as T7 RNA Polymerases. It plays a crucial role in various research and diagnostic workflows, including in vitro translation, RNA interference (RNAi), microinjection, probe hybridization, structure–function studies, and CRISPR-based applications.
Genaxxon offers a complete solution for your transcription needs – from high-yield RNA transcription kits to stand-alone enzymes (e.g., T7 RNA polymerase), and other essential reagents. Whether you need short or long transcripts, labeled RNA, or scalable RNA synthesis – we provide the tools to ensure reliable results.
Achieve efficiency, flexibility, and high RNA quality with our in vitro transcription products.
In vitro RNA transcription is a key method in molecular biology for synthesizing RNA from DNA templates using dedicated RNA polymerases such as T7 RNA Polymerases. It plays a crucial role in various research and diagnostic workflows, including in vitro translation, RNA interference (RNAi), microinjection, probe hybridization, structure–function studies, and CRISPR-based applications.
Genaxxon offers a complete solution for your transcription needs – from high-yield RNA transcription kits to stand-alone enzymes (e.g., T7 RNA polymerase), and other essential reagents. Whether you need short or long transcripts, labeled RNA, or scalable RNA synthesis – we provide the tools to ensure reliable results.
Achieve efficiency, flexibility, and high RNA quality with our in vitro transcription products.
GNX T7 RNA Polymerase - low dsRNA (250U/µL)
€2,295.00*
From
€543.00*
Optimized Enzyme for In Vitro TranscriptionGNX T7 RNA polymerase - low dsRNA is a recombinant enzyme expressed in Escherichia coli and is a genetically modified version of the wild-type T7. The mutation significantly reduces the formation of double-stranded RNA (dsRNA) during transcription, making it ideal for applications requiring high RNA purity.The polymerase synthesizes RNA in the 5'→3' direction using double-stranded DNA containing a T7 promoter sequence as a template. NTPs (ATP, CTP, GTP, UTP) serve as substrates. Both linear DNA with blunt ends or 5' overhangs can be used as templates – making this enzyme compatible with linearized plasmids and PCR products for efficient in vitro transcription.Key Benefits:Engineered for low dsRNA contaminationSuitable for both short and long RNA transcriptsCompatible with blunt-end and 5' overhang DNAHigh RNA yields with excellent specificity
Ideal for sensitive applications such as in vitro translation, RNAi, and vaccine researchThis polymerase is the perfect choice for precise, efficient, and clean RNA synthesis in molecular biology workflows.Synthesis Principle:
Variants from €543.00*
GNX T7 RNA Polymerase (250U/µL)
From
€473.00*
High-Purity Enzyme for In Vitro TranscriptionGNX T7 RNA Polymerase is a recombinant enzyme derived from bacteriophage T7 and expressed in Escherichia coli. It catalyzes the 5'→3' synthesis of RNA using double-stranded DNA containing a T7 promoter sequence (5'-TAATACGACTCACTATAGG-3') as a template and NTPs (ATP, CTP, GTP, UTP) as substrates for in vitro transcription.The polymerase is compatible with linear double-stranded DNA templates with either blunt ends or 5'-protruding ends. This makes it suitable for use with both linearized plasmid DNA and PCR products containing a T7 promoter.Supplied in a convenient liquid format, this enzyme is ideal for a variety of molecular biology applications such as RNA synthesis, RNA interference (RNAi), hybridization, in vitro translation, and the preparation of RNA probes.Key Features:High activity and specificity for T7 promoter sequencesSuitable for short and long RNA transcriptsCompatible with PCR products and linearized plasmids (blunt end or 5'-overhang)Liquid formulation for immediate useSynthesis principle:
GNX T7 High Yield RNA Synthesis Kit
€330.00*
€645.00*
€2,295.00*
From
€97.00*
Efficient in vitro Transcription with High RNA YieldThe T7 High Yield RNA Synthesis Kit provides an optimized transcription system for the efficient in vitro transcription of single-stranded RNA. Utilizing T7 RNA polymerase and a linear double-stranded DNA template containing the T7 promoter sequence, the kit enables the synthesis of 100–200 µg of RNA from just 1 µg of DNA template.Designed for both short and long RNA transcripts, this kit supports the incorporation of modified nucleotides, such as biotin- or dye-labeled NTPs, allowing for the generation of labeled RNA for downstream applications.Applications include:RNA structure and function studiesRNase protection assaysProbe hybridizationRNA interference (RNAi)MicroinjectionIn vitro translationKey benefits: High RNA yield (up to 200 µg per reaction) Suitable for long and short transcripts Easy incorporation of labeled nucleotidesReliable and reproducible results Versatile use in molecular biology and genetic research Synthesis principle:The ideal tool for researchers in molecular biology, genetics, and biotechnology.
Variants from €97.00*
Variants from €97.00*
Variants from €97.00*