Molecular Biology - Products for Molecular Biology Research

Agarose LE is a standard agarose for the separation of DNA in the size range between 100bp and 25kbp. It is suitable for all analytical and preparative electrophoresis of nucleic acids in routine gel electrophoresis. Depending on the concentration of Agarose LE used, the size range of nucleic acid separation will vary between 100bp and 25kbp. The low EEO makes this useful for a broad range of applications: PCR product analysis, restriction enzyme digest analysis, separation of RNA before blotting, etc. This agarose is comparable with, e.g. Agarose BioRagent, low EEO from Sigma or with the Universal-Agarose, peqGOLD from Peqlab. Free Taq DNA Polymerase test sample available! No shipping costs within Germany. Genaxxon offers Agaroses for different purposes. Two melting point options and normal or high resolution separation of DNA fragments are available. Standard normal melting agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 100bp up to 25kbp. This agarose is available as powder (M3044 Agarose LE >) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. They are compact, pre-weight and therefore easy-to-use. Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, agarase, as well as for "in-gel" DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel. High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®). For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.

Agarose LE tablets of 0.5g each for separation of DNA in the size range between 100bp and 25kbp. Agarose LE is suitable for all analytical and preparative electrophoresis of nucleic acids in routine gel electrophoresis. Depending on the concentration of Agarose LE used, the size range of nucleic acid separation will vary between 100bp and 25kbp. The low EEO makes this useful for a broad range of applications: PCR product analysis, restriction enzyme digest analysis, separation of RNA before blotting, etc.. Very easy to handle: No weighing No dust Easy to dissolve Comparable to SeaKem® LE. Genaxxon offers Agaroses for different purposes. Two melting point options and normal or high resolution separation of DNA fragments are available.Standard high melting point agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 50bp up to 50kbp. This agarose is available as powder (M3044 Agarose LE >) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. They are compact, pre-weight and therefore easy-to-use. Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, agarase, as well as for "in-gel" DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel. High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®). For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.

Agarose LM is the original low melting agarose. This "molecular biology grade" agarose gives gels with better properties and higher transparency than the standard normal melting point agarose. The agarose is equivalent to SeaPlaque™ from Lonza. Agarose LM is a low melting agarose with a very high separation capacity for large DNA fragments (>1000 bp), RNA and proteins. Enzymatic manipulations (eg, digestion, ligation, PCR, etc.) can be performed in the molten gel. Extraction of the DNA is therefore not necessary. Low melting Agarose LM is excellently suited for digestion with β-agarase (S5223). Large DNA fragments can be isolated very gently. The low melting agarose LM is used in the analytical and preparative field. For optimal results, the gel should be stored for at least 1 hour before use at + 2 ° C to + 8 ° C. Genaxxon offers agaroses for a wide variety of applications. There are agaroses with different melting points or with normal or high-resolution separation properties of DNA fragments. Standard melting point agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 50bp up to 20kbp. This agarose is available as powder (Standard Agarose LE (M3044 >)) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. These are compact, pre-weight and therefore easy-to-use. Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, Agarase (S5223) >, as well as for “in-gel’ DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel. High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®). For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.

Agarose Mega resolved DNA and RNA fragments from >100bp up to 50kb. Due to the high gel strength the agarose is ideally suited for Southern- and Northern blotting experiments or Pulsed Field Gel Electrophoresis (PFGE) as gel will not break during handling or electrophoresis. Due to the high gel strength, low concentrations of agarose may be used and high molecular weight molecules migrate rapidly without significant resctriction. The high gel strength reduces separation times for large DNA molecules while maintaining high resolution. Agarose Mega can also be used for the preparative separation of nucleic acids or for Ouchterlony or radial immundiffusion experiments. Genaxxon offers Agaroses for different purposes. Two melting point options and normal or high resolution separation of DNA fragments are available. Standard agarose high melting point agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 50bp up to 20kbp. This agarose is available as powder (M3044 Agarose LE >) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. These are compact, pre-weight and therefore easy-to-use. Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, Agarase, as well as for “in-gel’ DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel. High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®). For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.

Agarose Tiny is a low-melting high resolution agarose that is suitable for molecular biology applications and produces gels with better separation properties and greater clarity than standard agarose. It is very transparent even at high gel concentrations (3.0 - 4.5%) and can be used with all buffer systems. The low melting temperature makes Agarose Tiny ideal for preparative DNA and RNA electrophoresis, while the low gelling temperature is ideal for tissue culture cell cloning experiments and viral plaque assays. In addition to the above mentioned properties, Agarose Tiny can also be used for high-resolution (2 bp) gel electrophoresis in the range from 10 to 500 bp. Example: PCR products or small DNA fragments after restriction digestion or in mutation analyses. Features:Like Lonza's SeaPlaque™, Agarose Tiny has a low melting point (<64°C, 3.0% gel) and gel point (<25°C, 3.0% gel) and low DNA-binding capacity. Their use is particularly recommended for preparative RNA and DNA gels. Genaxxon offers Agaroses for different purposes. Two melting point options and normal or high resolution separation of DNA fragments are available. Standard agarose high melting point agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 50bp up to 20kbp. This agarose is available as powder (M3044 Agarose LE >) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. These are compact, pre-weight and therefore easy-to-use. Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, Agarase, as well as for “in-gel’ DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel. High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®). For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.

Our Agarose Tiny HT is a high resolution (+/-2 bp), normal melting agarose for fine resolution of small DNA fragments in the 20 bp to 1500 bp range. It can be used for genotyping, allele sizing and short tandem repeat analysis. Agarose Tiny works as a molecular screen and has twice the resolution of the finest sieving agaroses. It can discriminate between fragments which differ by only 2-4 bp in length and thus, is able to compete with acrylamide gels, being much easier to handle as the latter. Agarose Tiny HT 2% to 4% gels give similar results to Polyacrylamide 6% to 8% gels. For efficient resolution, the concentration of Agarose Tiny HT should be adjusted according to the range of band sizes analyzed, and the gel running buffer used (TAE or TBE). Genaxxon offers Agaroses for different purposes. Two melting point options and normal or high resolution separation of DNA fragments are available.Standard agarose high melting point agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 50bp up to 20kbp. This agarose is available as powder (M3044 Agarose LE >) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. These are compact, pre-weight and therefore easy-to-use. Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, Agarase, as well as for “in-gel’ DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel. High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®). For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.

Genaxxon’s DNA Library Prep Kit for Illumina is your universal solution for preparing high-quality libraries for sequencing on Illumina high-throughput platforms. Designed to convert fragmented DNA input ranging from 1 ng to 1 μg into sequencing-ready libraries, this kit offers unmatched efficiency and reliability for your sequencing projects.WorkflowKey Features:Wide Compatibility: Ideal for various sample types, including low-quality FFPE and cfDNA, as well as conventional DNA samples.High Efficiency: Delivers superior library conversion rates, ensuring maximum yield from your input DNA.Accurate Detection: Enables precise identification of low-frequency mutations, making it perfect for sensitive sequencing applications.Streamlined Workflow: Experience a seamless, four-step workflow that completes in approximately three hours—all within a single reaction tube. This process eliminates the need for intermediate purification and sample transfers, reducing the risk of handling errors and preserving the integrity of your samples. With DNA Repair Enzymes II, the kit effectively repairs a wide range of DNA damages, including nicks, gaps, and oxidized bases, ensuring the highest quality libraries for your sequencing needs. Applications: Whole-genome sequencing Whole-exome or targeted capture sequencing Amplicon sequencing ChIP sequencing Metagenome sequencing Methylation sequencing Optimized for IlluminaThe resulting libraries are fully compatible with standard Illumina® sequencing reagents and protocols, delivering exceptional performance for high-coverage deep sequencing, de novo sequencing, and whole-genome resequencing projects.Compatibility: This kit is fully compatible with Genaxxon’s Unique Dual Index primer pairs. For 24 reactions, use the UDI MidiSet (M4402). For 96 reactions, you can either use 2x UDI Set A (M4403) or 1x UDI Set A (M4403) and 1x UDI Set B (M4404).

Genaxxon’s DNA Library Prep Kit PLUS for Illumina is an advanced solution designed to enhance your sequencing workflow on Illumina high-throughput platforms. This kit simplifies DNA library preparation by integrating DNA fragmentation, end repair, and dA-tailing into a single step. With adapter ligation, library amplification, and size selection performed directly—without additional purification—the entire process is completed in just 2 hours.WorkflowKey Features:Efficient Workflow: Streamline your process by performing fragmentation, end repair, and dA-tailing with a single enzyme mix, finishing in under 2 hours.Versatile Insert Sizes: Customize your insert sizes by adjusting fragmentation time, while minimizing inversions and chimeras for optimal results.Broad Compatibility: Designed for diverse DNA sources, with strict background bacterial control for accurate pathogen detection.Optimized Performance: The Genaxxon DNA Library Prep Kit PLUS is compatible with DNA from various sources, including challenging samples like FFPE DNA. Enhanced reagent optimization reduces false positives, improving sequencing yield and overall results. This kit is particularly well-suited for constructing PCR-free libraries using 100 ng of high-quality genomic DNA. Applications: Whole-genome sequencing Exon or other targeted sequencing Metagenomic sequencing Optimized for IlluminaGenaxxon’s DNA Library Prep Kit PLUS delivers exceptional performance for a range of sequencing applications, ensuring your libraries are fully compatible with Illumina® sequencing reagents and protocols, making it the ideal choice for efficient, high-quality sequencing preparation.Compatibility: This kit is fully compatible with Genaxxon’s Unique Dual Index primer pairs. For 24 reactions, use the UDI MidiSet (M4402). For 96 reactions, you can either use 2x UDI Set A (M4403) or 1x UDI Set A (M4403) and 1x UDI Set B (M4404).

DNase I is an endonuclease isolated from bovine pancreas that digests double- and single-stranded DNA into oligo- and mono-nucleotides. DescriptionDNAse I is used, among other things, to avoid unwanted cell clumping during tissue disaggregation, which makes dispersion of individual cells difficult. Since tissue disaggregation and subsequent isolation of single cells is always accompanied by rupture and lysis of some cells, DNA is released from these cells into the surrounding medium. The released DNA in the medium is thought to be responsible for undesirable cell clumping during tissue dissociation procedures. This clumping effect can be avoided by adding deoxyribonuclease (DNase) to the dissociation medium and has been described for a variety of different cell and tissue types. DNase I is suitable for eliminating DNA from RNA preparations prior to sensitive applications, such as RT-PCR. Since no RNA purification procedure removes 100% of the DNA, RNA samples should be digested with DNase I before RT-PCR. A simple 15 minute digestion at room temperature removes the contaminating DNA. DNase I is inactivated by adding the stop solution and heating. Heating also denatures the RNA, so the RNA can be used directly for reverse transcription. This DNAse I is not RNase-free and has to be chemically treated if used for RNA isolation procedures.

GelRed(TM) is an ultra sensitive, extremely stable fluorescent dye designed to replace the toxic and possibly mutagenic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed(TM) is far more sensitive than EtBr without requiring a destaining step. GelRed(TM) is far less toxic and mutagenic compared to EtBr while both show virtually the same UV-spectra, so you can directly replace EtBr with GelRed(TM) without changing your existing imaging system. GelRed(TM) can be used to stain dsDNA, ssDNA or RNA in agarose gel via either precast or post gel staining. GelRed(TM) can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining. GelRed(TM) is also compatible with downstream DNA manipulations such as digestion with a restriction enzyme, Southern blotting techniques and clonings. A series of safety tests has confirmed that GelRed(TM) is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, GelRed(TM) can be safely disposed in regular trash, providing convenience and reducing cost in waste disposal. This fluorescent dye is supplied as a 10,000X solution in water. For customers who look for large pack size, we offer a cost-saving bulk pack size of 2mL, 5mL or 10mL (M3199.1010). For high-class agarose gels we offer the Genaxxon standard agarose LE > or our speciality agaroses for high resolution Tiny (M3046) > and Tiny HT (M3047) >.

The GenLadder 50bp (ready-to-use) is an ideal DNA size marker for fragment sizes from 50 - 1500bp. The bands at 200bp, 500bp and 1200bp bands light up more intensive for better orientation. Each band has been calibrated with respect to base pair size. Thus, an accurate determination of product size is possible. No ambiguous bands are visible. Band sizes: 50 (ca. 30ng/5µL), 100 (ca. 27ng/5µL), 150 (ca. 30ng/5µL), 200 (2x) (ca. 67ng/5µL), 250 (ca. 25ng/5µL), 300 (ca. 20ng/5µL), 350 (ca. 18ng/5µL), 400 (ca. 27ng/5µL), 450 (ca. 23ng/5µL), 500 (2x) (ca. 67ng/5µL), 600 (ca. 20ng/5µL), 700 (ca. 23ng/5µL), 800 (ca. 27ng/5µL), 900 (ca. 30ng/5µL), 1000 (ca. 33ng/5µL), 1200 (2X) (ca. 70ng/5µL) and 1500bp (ca. 40ng/5µL). GenLadder 50bp is already dissolved in loading buffer with tracking dye (Orange G) that does not overlab with bands in the range between 500bp and and smaller. GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.

Our DNA marker GenLadder 100 bp Plus with loading dye is an ideal tool to determine the size of double stranded DNA in the range of 100 - 3000 bp. The additional bands at 1.5kbp and 3.0kbp allow to assign additional DNA fragment sizes in the range up to 3000 bp without using an additional 1kbp DNA marker. The Gene Ladder 100 bp Plus consists of 12 fragments in sizes between 100 - 1000bp spaced 100bp apart. In addition, there are further bands at 1500bp and 3000bp. The 500bp and 1500bp fragment show stronger intensity to allow easier identification. All fragments are 'blunt-ended'. The GenLadder can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography. Fragment sizes (in base pairs): 3000 bp (40ng/5µL), 2x 1500 bp (70ng/5µL), 1000 bp (50ng/5µL), 900 bp (40ng/5µL), 800 bp (40ng/5µL), 700 bp (30ng/5µL), 600 bp (30ng/5µL), 2x 500 bp (90ng/5µL), 400 bp (40ng/5µL), 300 bp (30ng/5µL), 200 bp (40ng/5µL), 100 bp (40ng/5µL). The DNA marker is already dissolved in buffer containing orange G and Xylene cyanol FF as tracking dyes. The total concentration of the DNA-Marker is 0.1µg/µL (108µg/mL). The recommended amount of DNA marker per lane is 0,5μg (5µL). GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.

The GenLadder 100 bp Plus with gel staining dye and loading dye - ready-to-use is an innovation for high throughput approaches or colony screenings! Ideally suited to determine the size of double-stranded DNA between 100 and 1000 base pairs. The additional bands at 1.5kbp and 3.0kbp allow additional DNA sizes in this range to be assigned without having to use an additional 1kbp DNA marker. At the same time, the mixture also contains a fluorescent gel staining dye. Additional staining (pre- or post staining) or the addition of fluorescent dye prior to application is thus unnecessary and saves an additional pipetting step. At the same time, it is possible to check whether a PCR was successful or not before applying the PCR sample! This DNA ladder is an ideal match for our Red MasterMix Fluoro 2X, which also contains the gel staining dye. Together the ideal pair for Colony Screens! For more information on our SimplyEnlight PCR products, read our blog now. Genaxxon's SimplyEnlight PCR - Unlock the Power of Your PCR! The Gene Ladder 100 bp Plus consists of 12 fragments in sizes between 100 - 1000bp spaced 100bp apart. In addition, there are further bands at 1500bp and 3000bp. The 500bp and 1500bp fragment show stronger intensity to allow easier identification. All fragments are 'blunt-ended'. The GenLadder can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography. Fragment sizes (in base pairs): 3000 bp (40ng/6µL), 2x 1500 bp (70ng/6µL), 1000 bp (50ng/6µL), 900 bp (40ng/6µL), 800 bp (40ng/6µL), 700 bp (30ng/6µL), 600 bp (30ng/6µL), 2x 500 bp (90ng/6µL), 400 bp (40ng/6µL), 300 bp (30ng/6µL), 200 bp (40ng/6µL), 100 bp (40ng/6µL). The DNA marker is already dissolved in buffer containing orange G and Xylene cyanol FF as tracking dyes. The total concentration of the DNA-Marker is 90 µg/mL. The recommended amount of DNA marker per lane is 0,5μg (6µL). GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3084 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.

The GenLadder 1kb (ready-to-use) 250bp - 10kbp-ladder is the ideal DNA size marker for fragment sizes from 250 - 10000bp. All bands light up to the same intensity except the 1000 bp and the 3000 bp band which are twice as bright for better orientation. Each band has been calibrated with respect to base pair size and DNA quantity. Thus, an accurate determination of product size and DNA concentration is possible. No ambiguous bands are visible. The GenLadder 250bp - 10kb can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography. The DNA is already dissolved in buffer with 6X loading dye ready to use. Fragment sizes and amount of DNA per band: 10000bp ( 28ng/5µL), 8000bp (28ng/5µL), 6000bp (28ng/5µL), 5000bp (28ng/5µL), 4000bp ( 18ng/5µL), 2x3000bp (92ng/5µL), 2500bp (34ng/5µL), 2000bp (34ng/5µL), 1500bp (20ng/5µL), 2x1000bp (92ng/5µL), 750bp (23ng/5µL), 500bp (30ng/5µL), 250bp (45ng/5µL). GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.

With the synthetic GenLadder XLarge, even larger plasmid insertions and vector plasmids can be determined clearly and precisely in size by gel electrophoresis in the range of 250 - 25000 base pairs after restriction digests. The Genaxxon GenLadder XLarge shows 14 bands. The approximate mass of the DNA in each band is given for a 5µL load (0.52µg/lane). Additionally the 1000 bp and 3000 bp are intensified for better and faster orientation in 1.0 - 1.5% agarose gels. Please avoid repeated freez-thaw cycles. We recommend to portion the product into smaller aliquots. Source: PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10mM Tris-HCl (pH8.0) and 10mM EDTA. GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.

Restriction enzyme-digested lambda DNA (48,502bp) generates molecular weight size markers routinely used in gel analysis of nucleic acids. The complete nucleotide sequence has been determined. Lit.: Sanger, F. et al. (1982) J. Mol. Biol. 162, 729. Details about the DNA sequence can be found in the data sheet attachement. Description/Preparation: The double stranded DNA is isolated from bacteriophage lambda (cl857 ind1 Sam7). The molecular weight is 31.5 x 10E6 daltons and is 48,502 base pairs in length.The phage is isolated from the heat inducible lysogen E. coli λ cl857 S7 by gel filtration. The DNA is isolated from the purified phage by phenol/chloroform extraction and dialyzed against 10mM Tris-HCl (pH7.4) and 1mM EDTA. The product is methylated. GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye which contains bromophenol blue and xylene cyanol FF): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.

Die Vektoren pUC18/ pUC19 aus E.coli sind standardmäßig benutzte, kleine high copy number E. coli Plasmide von 2686 bp Länge. pUC19 ist identisch mit pUC18, außer dass die multiple cloning site (MCS) in umgekehrter Orientierung angeordnet ist. pUC18/19 Plasmide beinhalten: 1. das pMB1 Replikon rep, das für die Replikation des Plasmids zuständig ist (Quelle – Plasmid pBR322). Die hohe Kopienzahl des pUC Plasmids resultiert aus dem Fehlen des rop-Gens und einer Punktmutation im Replikon rep von pMB1. 2. das bla-Gen, das für die beta-Laktamase kodiert und die Resistenz gegen Ampicillin (Quelle – Plasmid pBR322) vermittelt. Es unterscheidet sich vom bla-Gen aus pBR322 durch 2 Punktmutationen. 3. die Region des E.coli lac-Operons, das die CAP-Protein Bindestelle, den Promotor Plac, die lac-Repressor Bindestelle und den 5’-terminalen Teil vom lacZ-Gens vermittelt, das für das N-terminale Ende der beta-Galaktosidase (Quelle – M13mp18/19) kodiert. Dieses Fragment, dessen Synthese durch IPTG induziert werden kann, ist zu einer intra-allelen (alpha) Komplementation mit einer vom Wirt kodierten, defekten Form der beta-Galaktosidase befähigt (Mutation - delta(lacZ)M15). In der Gegenwart von IPTG synthetisieren die Bakterien beide Fragmente des Enzyms und bilden blaue Kolonien auf Medien, die X-Gal enthalten. Die Insertion von DNA in die MCS, die inmitten des lacZ Gens liegt (Codons 6-7 des lacZ Gens wurden durch die MCS ersetzt), inaktiviert das N-terminale Fragment der beta-Galaktosidase und verhindert die alpha-Komplementation. Die Bakterien, die rekombinante Plasmide enthalten, bilden deshalb weiße Kolonien auf Medien mit X-Gal. GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye which contains bromophenol blue and xylene cyanol FF): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.

NGS DNA Puri MagBeads is a SPRI-based chemistry that speeds and simplifies nucleic acid size selection and PCR purification for DNA and/or RNA library preparation for Next Generation sequencing. It is compatible with mainstream NGS library prepared kits. NGS DNA Puri MagBeads utilize SPRI (Solid Phase Reverse Immobilization) paramagnetic bead technology to enable DNA purification and size selection during NGS library construction.NGS DNA Puri MagBeads are compatible with most NGS library construction kits (DNA, RNA). The kit allows users to seamlessly replace competitor beads (i.e. AMPure XP Beads) in current bead clean-up and size selection protocols without loss of efficiency. Why Purification and Quality Control of DNA preps for NGS?The purification step is usually necessary to remove unwanted material that might interfere with sequencing. Some NGS platforms may have tight size requirements, and discarding fragments that are too large or too small can improve sequencing efficiency by far. The optimal library size is given by the sequencing application. It is common to clean up nucleic acids using magnetic beads. Quality control is the last process before sequencing. As Subsequent experiments are time-consuming and expensive, strict quality control steps are required to ensure all samples are appropriate for their applications. Confirming the quality and quantity of DNA increases the reliability of sequencing data! The Genaxxon NGS MagBead DNA purification kit protocol can be adapted to your current liquid handling workstation (e.g., Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent and Eppendorf) utilizing your current protocol as well as it can be performed manually.

Overview:Proteinase K is a robust and versatile serine protease used extensively in molecular biology for the digestion of proteins in nucleic acid preparations. This solution, at a concentration of 20 mg/mL, is ideal for a wide range of applications including the preparation of DNA and RNA samples. The PCR grade quality ensures that the enzyme is free from contaminants that could interfere with downstream applications, making it highly suitable for sensitive techniques such as PCR.Product Details:ready-to-useConcentration: 20 mg/mLactive over a wide range of reaction conditionsAn elevation of the reaction temperature from 37°C to 50 - 60°C may increase the activity several timesQuality: PCR grade, ensuring high purity and no interference with PCR reactions.Applications: Suitable for DNA and RNA extractionRemoval of protein contaminantsPreparation of samples for various molecular biology techniques.Benefits:High Purity: The PCR grade quality guarantees minimal contamination, ensuring reliable results in sensitive applications. Versatility: Ideal for various molecular biology procedures, including sample preparation for PCR.Convenience: Ready-to-use solution at an optimal concentration for ease of use. Notes:The recommended working concentration of Proteinase K is 0.05 to 1 mg/mL. The activity of the enzyme is stimulated by 0.2 to 1% SDS and also by 1 to 4M ureaCa2+ protects Proteinase K against autolysis and increases the thermal stabilityStable over a wide pH range: 4.0 to 12.5, optimum pH 7.5 to 8.0Activity optimum: 50°C to 55°CRapid denaturation of enzyme occurs at temperatures above 65°CFeatures:No detected exonuclease, endonuclease or RNase activitySpecific activity: >40 units/mg protein Activity: ≥30 units/mg lyophilizateSolubility: ≥20 mg/mLDNA content ≤10 pg/mgShipping conditions: Shipment on wet iceProteinase K can be obtained from Genaxxon as Powder (M3036) > or as 20mg/mL Solution (M3037) >.Produced by Genaxxon bioscience GmbH, founded in 2002 by Dr. Norbert Tröndle to provide reliable products for PCR and custom cell culture media formulations, the Proteinase K Solution 20 mg/mL offers a dependable and efficient solution for your molecular biology needs. Achieve consistent, high-quality results with an enzyme designed specifically for your requirements.

Overview:Proteinase K is a robust and versatile serine protease used extensively in molecular biology for the digestion of proteins in nucleic acid preparations. This solution, at a concentration of 20 mg/mL, is ideal for a wide range of applications including the preparation of DNA and RNA samples. The PCR grade quality ensures that the enzyme is free from contaminants that could interfere with downstream applications, making it highly suitable for sensitive techniques such as PCR.Product Details:ready-to-useConcentration: 20 mg/mLactive over a wide range of reaction conditionsAn elevation of the reaction temperature from 37°C to 50 - 60°C may increase the activity several timesQuality: PCR grade, ensuring high purity and no interference with PCR reactions.Applications: Suitable for DNA and RNA extractionRemoval of protein contaminantsPreparation of samples for various molecular biology techniques.Benefits:High Purity: The PCR grade quality guarantees minimal contamination, ensuring reliable results in sensitive applications. Versatility: Ideal for various molecular biology procedures, including sample preparation for PCR.Convenience: Ready-to-use solution at an optimal concentration for ease of use. Notes:The recommended working concentration of Proteinase K is 0.05 to 1 mg/mL. The activity of the enzyme is stimulated by 0.2 to 1% SDS and also by 1 to 4M ureaCa2+ protects Proteinase K against autolysis and increases the thermal stabilityStable over a wide pH range: 4.0 to 12.5, optimum pH 7.5 to 8.0Activity optimum: 50°C to 55°CRapid denaturation of enzyme occurs at temperatures above 65°CFeatures:No detected exonuclease, endonuclease or RNase activityConcentration ≥20 mg/mL / Activity ≥800 U/mLDNA content ≤200 pg/mLShipping conditions: Shipment on wet ice Storage buffer: 10 mM Tris/HCl, pH7.5; 1mM (CH3COO)2Ca; 50% glycerolProteinase K can be obtained from Genaxxon as Powder (M3036) > or as 20mg/mL Solution (M3037) >.Produced by Genaxxon bioscience GmbH, founded in 2002 by Dr. Norbert Tröndle to provide reliable products for PCR and custom cell culture media formulations, the Proteinase K Solution 20 mg/mL offers a dependable and efficient solution for your molecular biology needs. Achieve consistent, high-quality results with an enzyme designed specifically for your requirements.

Genaxxon Proteinase Low-Temp is a nonspecific endopeptidase derived from an Arctic marine microbial source. It has a broad substrate specificity and is easy to inactivate after use. Starting at a temperature of 60°C, the Genaxxon Proteinase Low-Temp is heat-inactivated, making it ideal for heat-sensitive samples. The Proteinase Low-Temp is most active in the temperature range of 25°C-40°C. Recommended inactivation conditions are: Typically up to 15 minutes at 40°C – 65°C (inactivation by denaturation). It is known that histones and other proteins shield nucleic acids from an optimal interaction with other DNA-binding proteins and enzymes. Genaxxon Proteinase Low-Temp is ideal for transforming chromatin and other compact nucleic acids into mere DNA. The enzyme is easily inactivated. This allows thermal inactivation at temperatures that provide RNA integrity and avoid the dissociation of dsDNA.Advantages:- Easy to deactivate- Active at high salt content- Compatible with downstream analyzes Application examples:Preparation of chromosomal DNA for PFGE- Degradation of nucleases (DNase, RNase) in the preparation of nucleic acids, including the recovery of native RNA- Isolation of genomic DNA from tissue of mouse tails- Isolation of genomic DNA from cell culturesSpecific activity: >30 U/mg. Foreign activity: RNase and DNase undetectable. Temperature optimum: + 25°C-40°C (pH 7-10). Recommended inactivation conditions: Typically up to 15 minutes at 40°C – 65°C (inactivation by denaturation).Inhibitors: General Serine protease inhibitors such as PMSF (M3194) >.Instructions for use: The enzyme is stable up to 2 years when stored at -20°C. Genaxxon Proteinase Low-Temp is stable even when stored at 4°C (>6 months) and room temperature (>4 weeks).The optimal enzyme activity is between pH 7 and 10. Recommended temperature range for the activity is 25-40°C. The activity of Genaxxon Proteinase Low-Temp is not dependent on divalent cations such as calcium (Ca2 +) and is therefore active in buffers containing EDTA (<40°C reaction temperature).Genaxxon Proteinase Low-Temp is suitable for the digestion of proteins in the presence of SDS (0.2-1%) and urea (1-5 M). The enzyme tolerates 500mM guanidine thiocyanate and 1% Triton X-100 (>50% activity). There is no significant loss of activity at 400 mM NaCl. Proteinase K is used for the digestion of proteins in cell lysates (tissue, cell culture cells) and for the release of nucleic acids, since it very effectively inactivates DNases and RNases. The digest with Proteinase K for the purification of nucleic acids is performed in the presence of EDTA (inhibition of magnesium-dependent enzymes). Proteinase K can be obtained from Genaxxon as Powder (M3036) > or as 20mg/mL Solution (M3037) >.

Ribonuclease A is an endo-ribonuclease specifically cleaving single stranded RNA at the 3' side of pyrimidine bases (cytosine and uracil). RNAse A ist used to prepare RNA-free DNA, to digest non-hybridised regions of RNA-DNA hybrids and as a molecular weight marker. The pH optimum for RNAse A is between 7.0-7.5. RNAse A is inhibited by Diethylpyrocarbonate (DEPC) >, guanidinium salt (4M GuaSCN), beta-Mercaptoethanol, heavy metals, vanadyl-ribonucleoside complexes, RNAse-inhibitor from human placenta and by competitive DNA. RNAse A cleaves single- and double-stranded RNA and RNA in RNA:DNA hybrids at low salt conditions (100 mM NaCl). At high salt conditions (>300 mM NaCl), RNAse A cleaves single-stranded RNA only. The enzyme can only be removed by Proteinase K digest and subsequent phenol extraction. Elimination of DNAse activity: Product S5231 (RNAse A (not certified DNAse free)) may contain DNAse activity. As RNAse A is heat stable it is recommended to heat inactivate DNAse activity before use. Procedure: 10mg/mL RNAse A in 0.01 sodium acetate (pH5.2) have to be heated to 100°C for 15 minutes in a water bath. After 15 minutes, the RNAse shall remain in the water bath while the water cools down to room temperature. pH can be adjusted by addition of 0.1 time the volume of 1M Tris-HCl (pH7.4). After aliquotation of the now DNAse free RNAse A solution, each aliquote should be stored a -20°C. NOTE: RNAse A precipitates if concentrated solutions are heated to 100°C at neutral pH! Stock solution: Stock solutions are prepared at concentrations from 1 - 10mg/mL in 10mM Tris/HCl, pH7.5; 15mM NaCl or in 10mM Tris/HCl, pH7.5; 1mM EDTA, pH8.0 (TE buffer).The recommended working concentration is 10µg/mL (removal of RNA from plasmid preparations; 1hr, RT) or 100ng/mL (preparation of 'blunt ends' of double-stranded cDNA). Stability:RNase A aggregates during lyophilizing and storage. It has a high affinity to glas surfaces, which has to be taken into consideration. At neutral pH (e. g. in PBS pH 7.4) and high concentrations (>10mg/mL) the enzyme will precipitate. At +2°C to +8°C (lyophilized) it is stable for several years (dry storage), in solution (-20°C) several years or (+2°C to +8°C) several weeks.

Ribonuclease A- DNase-free is a DNase and Protease-free enzyme that can be used directly for RNA digests, e.g. in DNA purification procedures. Elimination of potential DNAse activity is not necessary. Potential DNAse activities have been removed. DescriptionRibonuclease A is an endo-ribonuclease specifically cleaving single stranded RNA at the 3' side of pyrimidine bases (cytosine and uracil). RNAse A ist used to prepare RNA-free DNA, to digest non-hybridised regions of RNA-DNA hybrids and as a molecular weight marker. The pH optimum for RNAse A is between 7.0-7.5. RNAse A is inhibited by Diethylpyrocarbonate (DEPC) >, guanidinium salt (4M GuaSCN), beta-Mercaptoethanol, heavy metals, vanadyl-ribonucleoside complexes, RNAse-inhibitor from human placenta and by competitive DNA. RNAse A cleaves single and douple stranded RNA and RNA in RNA:DNA hybrids at low salt conditions (100mM NaCl). At high salt conditions (>300 mM NaCl), RNAse A cleaves single-stranded RNA only. The enzyme can only be removed by Proteinase K digest and subsequent phenol extraction. Stock solution: Stock solutions are prepared at concentrations from 1 - 10mg/mL in 10mM Tris/HCl, pH7.5; 15mM NaCl or in 10mM Tris/HCl, pH7.5; 1mM EDTA, pH8.0 (TE buffer).The recommended working concentration is 10µg/mL (removal of RNA from plasmid preparations; 1hr, RT) or 100ng/mL (preparation of 'blunt ends' of double-stranded cDNA). Stability:RNase A aggregates during lyophilizing and storage. It has a high affinity to glas surfaces, which has to be taken into consideration. At neutral pH (e. g. in PBS pH 7.4) and high concentrations (>10mg/mL) the enzyme will precipitate. At +2°C to +8°C (lyophilized) it is stable for several years (dry storage), in solution (-20°C) several years or (+2°C to +8°C) several weeks.

SafeGel green stain is an ultra sensitive, extremely stable fluorescent dye designed to replace the toxic and possibly mutagenic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. SafeGel green stain is far more sensitive than EtBr without requiring a destaining step. SafeGel green stain is far less toxic and mutagenic compared to EtBr while both show virtually the same UV-spectra, so you can directly replace EtBr with SafeGel green stain without changing your existing imaging system. SafeGel can be used to stain dsDNA, ssDNA or RNA in agarose gel via either precast or post gel staining. SafeGel can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining. SafeGel is also compatible with downstream DNA manipulations such as digestion with a restriction enzyme, Southern blotting techniques and clonings. A series of safety tests has confirmed that SafeGel is noncytotoxic, nonmutagenic and nonhazardous even at concentrations above the working concentrations used in gel staining. As a result, SafeGel can be safely disposed in regular trash, providing convenience and reducing cost in waste disposal. This fluorescent dye is supplied as a 10,000X solution in water. For customers who look for large pack size, we offer a cost-saving bulk pack size of 2mL, 5mL or 10mL (M3193.1010). For high-class agarose gels we offer the Genaxxon standard agarose LE > or our speciality agaroses for high resolution Tiny (M3046) > and Tiny HT (M3047) >. * * *Discover our NEW Product: More Info PDF >

SafeGel red stain is an ultra sensitive, extremely stable fluorescent dye designed to replace the toxic and possibly mutagenic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. SafeGel red stain is far more sensitive than EtBr without requiring a destaining step. SafeGel red stain is far less toxic and mutagenic compared to EtBr while both show virtually the same UV-spectra, so you can directly replace EtBr with SafeGel red stain without changing your existing imaging system. SafeGel can be used to stain dsDNA, ssDNA or RNA in agarose gel via either precast or post gel staining. SafeGel can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining. SafeGel is also compatible with downstream DNA manipulations such as digestion with a restriction enzyme, Southern blotting techniques and clonings. A series of safety tests has confirmed that SafeGel is noncytotoxic, nonmutagenic and nonhazardous even at concentrations above the working concentrations used in gel staining. As a result, SafeGel can be safely disposed in regular trash, providing convenience and reducing cost in waste disposal. This fluorescent dye is supplied as a 10,000X solution in water. For customers who look for large pack size, we offer a cost-saving bulk pack size of 2mL, 5mL or 10mL (M3193.1010). Read in our blog why you should use Genaxxon's SafeGel now. For high-class agarose gels we offer the Genaxxon standard agarose LE > or our speciality agaroses for high resolution Tiny (M3046) > and Tiny HT (M3047) >.