Polyethylene glycol 8000 - PEG 8000
Advantages at a glance
- MW = 7000-9000 g/mol
- pH (5%, H2O): 4.5-7.5 (20°C)
- Solubility (5%, H2O): clear, colourless
- n(OH): 12 - 16
€55.63
- Free shipping
- Shipping within 24h
- 30 days money back guarantee
- Purchase comfortable on account
Delivery time: 3-8 days
Product information "Polyethylene glycol 8000 - PEG 8000"
PEG 8000 also known as Polyethylene glycol 8000 has a pronounced hydration capacity and is structurally flexible. Due to its biocompatibility and the absence of steric hindrances in its structure, PEG is often added to protein containing solution to stabilize the protein or to increase the solubility of the protein.
In molecular biology applications Polyethylene glycol 8000 is used as buffer component for transformation of yeast, or for buffers to isolate or to concentrate viruses.
Cloning:
Especially for the cloning with 'blunt-ended' DNA, the concentration of 'blunt'-DNA-ends plays a crucial role for the successful cloning. Substances, that
increase the so-called 'macromolecular crowding' concentrate the DNA locally and increase the possibility, that DNA ends will meet and thereby improve the cloning result.
A final concentration of 5-15% PEG 8000 is often recommended for ligation reactions, but the optimal concentration can vary depending on the specific reaction and enzyme used. E.g. the kinetic of the ligation in the cloning of DNA-fragments into
bacteriophage M13-vectors can be improved by the inclusion of 5% polyethylene glycol 8000 (PEG 8000).
Prevents premature circularization:
While a 5% concentration is often optimal, exceeding 10% can inhibit transformation efficiency.
Stock solutions of PEG 8000 (40%) are prepared with deionized nuclease-free water and stored at -20°C in small aliquots.
Specifications:
MW = 7000-9000 g/mol
pH (5%, H2O): 4.5-7.5 (20°C)
Solubility (5%, H2O): clear, colourless
Heavy metals (as Pb): max. 0.005%
n(OH): 12 - 16
HO(C2H4O)nH
Klassifizierungen / Classification
Documents:
Safety Data SheetCertificate
Category List