50X Tris-Acetate-EDTA buffer (pH8.3 - 5 bags)

Advantages at a glance

Pre-mixed buffer powder with pre-set pH
Eliminates calculation, formulation and weighing errors
Dissolve-and-go for greater convenience
Stable at room temperature for at least 3 years
Guaranteed reproducibility

€357.21*

Volume

1 L

500 mL

Product number: D2029.0505
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.

Shipment: not cooled. Store at +15°C to +30°C. For laboratory usage only!

Product information "50X Tris-Acetate-EDTA buffer (pH8.3 - 5 bags)"

Contents of 1 pouch dissolved in deionized water and made up to 500mL/1000mL yields: 2.0M Tris acetate buffer, 0.05M EDTA, pH8.3 at 25°C. In molecular biology, TBE and TAE buffers are used for agarose and polyacrylamide gel electrophoresis.TBE buffer is suitable when analysing DNA fragments from PCR amplification, DNA isolation protocols, or DNA cloning experiments. It is adapted for separating smaller DNA fragments (less than 1500 bp on a 0.8% agarose gel). TAE is advantageous for high resolution of long nucleic acid fragments (longer than 1500 bp) on agarose gels. It has a lower buffering capacity than TBE and in general, nucleic acid fragments move slower in TAE gels (apart from linear dsDNA, which tends to run faster). TBE has a greater buffering capacity and will give sharper resolution than TAE. However, TBE gels in general afford a poor recovery of nucleic acids compared with TAE gels. TAE is also used for native (non-denaturing) RNA analysis and in denaturing gels (instead of MOPS buffer) using prior denaturation of the RNA samples in hot formamide. Medicago’s TBE and TAE buffers are supplied as a pre-weighed powder mix in sealed pouches giving 1000mL of 1X, 5X or 10X Tris-borate-EDTA buffer or 50X Tris-acetate-EDTA buffer with pH 8.3 at 25°C.

Specifications:
exactly pre-weight powder mix
concentration: 2M Tris/acetate, 0.05M EDTA, pH8.3 +/-0.1 (25°C)

Rnases/Dnases: not detectable.

Applikation / Application:

Einheiten / Units:

Quelle / Source:

synthetic

Sicherheits Hinweise / Safety

H-Sätze: H315, H319
GHS-Symbole: GHS07

Klassifizierungen / Classification

eclass-Nr: 32-16-04-03

Documents - Protocols - Downloads :

Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

Documents:

Safety Data Sheet
General Data 1
General Data 2
Category List

Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed