Bluo-Gal / 5-Bromo-3-indolyl-beta-D-galactopyranoside
Advantages at a glance
- for identification of lac+ bacterial colonies.
- An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal)
- producing a darker blue color compared to X-Gal
€143.98
- Free shipping
- Shipping within 24h
- 30 days money back guarantee
- Purchase comfortable on account
Delivery time: 3-8 days
Product information "Bluo-Gal / 5-Bromo-3-indolyl-beta-D-galactopyranoside"
5-Bromo-3-indolyl-β-D-galactopyranosid (Bluo-Gal) ist used as a substrat of β-Galactosidase. It is a chromogenic substrate suitable for identification of lacZ+ bacterial colonies. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Principle
When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.
Documents:
Safety Data SheetCertificate
Category List
Source: NCBI PubMed