IPTG, dioxane free, Molbio Grade
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Ready to ship today,
Delivery time 1-2 workdays
Shipment: not cooled. Store at +2°C to +8°C. Protect from light. For laboratory usage only!
IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used together with X-Gal > (5-bromo-4-chloro-3-indolyl-ß-D-galactoside) or Bluo-Gal > (5-Bromo-3-indolyl-beta-D-galactopyranoside) in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli.
Mode of operation:
While IPTG binds to the lacI repressor it is altering the conformation of the lacl repressor, which prevents the repression of the β-galactosidase coding gene lacZ. Thus IPTG is an inducer of the lac operon in bacteria for detection of lac+ colonies.
Dissolve in water to e.g. 200mg/mL, sterilise by filtration and store in aliquots at -20°C. For detection of transformants, use in final concentration of 0.1mM up to 1mM in LB-Medium.
Specifications:
Purity: min. 99% (HPLC)
Appearance: white powder
RNases, DNases: not detected
MP = 110-112°C
Specific rotation (a20°/D, c=1 water): -20.0° - -34.0°
Water: max. 0.5%
Dioxane: 1.4-Dioxane not detected
Inhibitor free: tested active in Lac-gene expression
C9H18O5S
MW = 238.3 g/mol
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
EC-Nr: 206-703-0CAS-Nr: 367-93-1
eclass-Nr: 32-16-04-90
Documents:
Safety Data SheetCertificate
Category List
Source: NCBI PubMed
