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Sanger sequencing technology was the first generation of sequencing and helped provide basic knowledge about genome sequencing but didn’t have the potential for its further improvement. Hence a new technological jump in sequencing expertise was required. That’s when Next Generation Sequencing techniques came into existence and revolutionized our entire capability of genome sequencing.
SNP PolTaq DNA polymerase for better Sake taste and flavor: SNP PolTaq for qPCR for the unambiguous identification of point mutations in yeast!
Read more about a recent case report from Japan.
Point mutations are the cause of many hereditary diseases and are considered risk factors, for example, for Alzheimer's Disease.
One of the main causes of many hereditary diseases could not be resolved precisely enough by mid-2016, even with the "super tool" CRISPR / Cas9: the point mutation.