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Standard PCR amplification once required often laborious DNA extraction, purification, end-stage processing and sample set-ups, using relatively primitive instruments and complex protocols. The notion that 35 cycles of PCR amplification could be completed in under 25 minutes, without compromising precision or accuracy, was considered a distant prospect.
Nowadays there are many different suppliers and systems for polymerase chain reaction (PCR) and of its derivative techniques, quantitative, or real-time, PCR (qPCR) and reverse transcription PCR (RT-PCR). Competition gives you the opportunity to simplify diagnostics and to make research more financially favorable.