Blog

Problems with non-specific bands after your PCR? Or, the PCR does not work, but you find lots of amplified primer dimers? No desire for hectic and cooling when pipetting your PCR and the following steps? Looking for reproducible results summer and winter? Then simply use a hot-start PCR.
Maybe you already know it: Even if you did not make a mistake when pipetting your PCR, the results can sometimes be frustrating and disappointing. But why? Conventional DNA polymerases are already active at room temperature - and this can lead to many "falsely amplified" templates, false-positive results and low yield.