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Extraction and isolation of viral RNA is the first step in the detection and testing of viral RNA by real-time RT-PCR. Here we compare different methods and protocols for extraction of viral RNA.
Nested RT-PCR and real-time RT-PCR are the most common methods for the analysis of viral infections (see Robert Koch Institute >). In contrast to examinations with ELISA or IFA, this can be done at an earlier stage of infection.