To achieve successful qPCR result is as important as it is to have a reliable sample to work with.
It is equally important to choose the right mastermix, but given the enormous market of mastermixes, choosing the one that caters to your needs can be overwhelming.
That is why Genaxxon has the best list of advantages that we can offer to you. Depending on your needs, you can choose the right customized mastermix for your use!
IIn this blog, I will discuss the GreenMasterMix FAST FAST without ROX for qPCR and ProbeMasterMix FAST FAST without ROX for qPCR.
GreenMasterMix FAST without ROX for qPCR without ROX for qPCR. Die Quantifizierung mit dem ProbeMasterMix FAST without ROX for qPCR
erfolgt zum Beispiel über TaqMan Sonden oder über Molecular Beacons Reportersonden
These two mastermix cocktails however have one thing in common: HotStart Taq DNA polymerase!
This HotStart technology enables:
1) Setup of PCR mixture at room temperature.
2) Short initial denaturing time of not more than 2 minutes.
3) Pipetting on ice is no longer necessary.
4) No immediate further processing (PCR) is necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days.
5) Amplification of GC-rich templates.
6) High yields.
7) No Primer dimers.
Want to save time and cut down on real time protocol steps? No problem!
Sie können die PCR Zeiten weiter verkürzen, indem sie Primer Annealing und Extension in einem Schritt kombinieren, wenn ihre Temperaturen innerhalb weniger Grad voneinander liegen. Dieses Verfahren wird auch als 2-Schritt PCR Protokoll bezeichnet.
Der GreenMasterMix (2x) without ROX for qPCR and ProbeMasterMix (2X) without ROX for qPCR have also been tested on LighCycler96 (Roche) using fast cycling real-time 2-step protocols; Fast PAH12 Green LC bzw. Fast Pthr Probe LC.
There are numerous researchers that have successfully incorporated our ProbeMasterMix FAST without ROX for qPCR into their research work.
A few of them are: Assessment of Fetal Rhesus D and Gender with Cell-Free DNA and Exosomes from Maternal Blood
Did you know that it might be possible to safely gather genomic information of the fetus during pregnancy?
Yes, using Exosomes!
A recent study using GENAXXONs ProbeMasterMix FAST ohne ROX für qPCR for RHD- and SRY-PCR-amplifications, showed that maternal Cell-Free DNA and Exosomes can reveal the baby’s gender and can also be used for prenatal studies. This would be the non-invasive approach for gathering genomic information of the fetus during pregnancy.
Our GreenMasterMix FAST ohne ROX für qPCR has been incorporated in following research studies:
1) Gold Nanoparticles Conjugated L- lysine for Improving Cisplatin Delivery to Human Breast Cancer Cells
The drug Cisplatin, commonly used for Cancer treatment, when conjugated to Gold nanoparticle conjugated to L-lysine resulted in a significant apoptosisof breast cancer cell line SKBR3 along with insignificant cytotoxicity on control cells. Also, the Lethal dose of Cisplatin required to induce this effect was significantly lower than that of naked Cisplatin. This study carried out q-RT-PCR to determine caspase 3 expression using GENAXXON GreenMasterMix FAST without ROX for qPCR (M3150). This study revealed that gold nanoparticles can exponentially increase the uptake of Cisplatin by Human Breast Cancer cells.
2) Can Biomarkers Respond Upon Freshwater Pollution?—A Moss-Bag Approach
RT-qPCR was performed using GreenMasterMix FAST ohne ROX für qPCR in a study where effect of contamination (heavy metals and overall poor water quality) in standing water bodies were studied for 30 days using Moss-bags. Alive Fontinalis antipyretica were exposed to the water and supressed gene expression of rbcL, declined TPC (Total phenolic content) was found in them. Interestingly, an increase in cell number was observed, while the cell size decreased. These results suggest that aforementioned biomarkers can be used as biomonitoring tools.
3) Immunomodulatory role of reactive oxygen species and nitrogen species during T cell-driven neutrophil-enriched acute and chronic cutaneous delayed-type hypersensitivity reactions
This research focused on the production, biochemical characteristics and consequences of ROS/RNS (in vivo and ex vivo) in wild type and T cell dependent acute and chronic TNCB (Trinitrochlorobenzene) induced cutaneous DTHR (Delayed type hypersensitivity reaction). This research was conducted to stud the effect of various anti-inflammatory sources on the development of T cell- driven neutrophil-enriched cutaneous immune responses. It was revealed that NADPH oxidase, the primary source of ROS/ RNS has an anti-inflammatory role in acute DTHR and the deficiency of myeloperoxidase (MPO), which turns superoxide to HOCl (hypoclorous acid) may have a role in promotion of chronic cutaneous DTHR.
4) MicroRNA profiling the resurrection plant Haberlea rhodopensis unveils essential regulators of survival under severe drought
This study provides evidence that miRNAs can help enhance drought induced transcriptome reprogramming efficiency in H.rhodopensis.
The miR156/157 and miR399 families are able to control plant development and growth by modulating transcription factor expression and therefore are essential for plant survival under severe water stress.