G418 - 50mg/mL
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Product information "G418 - 50mg/mL"
G418 also known as Geneticin(TM), is an aminoglycoside antibiotic that inhibits protein synthesis in mammalian cells by interfering with ribosomal function. G418 shows a structure similar to gentamycin > and kanamycin > and finds application in the selection of stably transformed cells expressing the neomycin resistance gene (aminoglycoside) of transposons Tn 5 or Tn 601.
Because G418 is toxic for many cells, the optimum concentration for each cell type has to be determined. It is usually between 50ug/mL and 1000μg/mL. At a concentration of 500μg/mL, for example, 70% of HepG2 cells are killed after incubation for one week and 100% will stop their growth. Since potency of the antibiotic can vary from batch to batch, it is recommended to create large stocks of a batch tested to standardize the system.
Cells divide still once or twice in the presence of the lethal dose of G418, so that a selection effect will be visible only after several days. Geneticin is a registered trademark of Gibco, a Division of Life Technologies.
Genaxxon's G418 is an alternative to ThermoFisher / Invitrogen product no. 10131035 and 10131027, or a replacement for BioChrom product no.: A 2812.
Genaxxon bioscience offers a broad range of Antibiotics and Antimycotics as powder or solutions.
Specifications:
50 mg/mL sterile filtered solution
C20H40N4O10x2H2SO4
MW = 692.7 g/mol
Liquid formulation of Cat#: M3117
Harmful: LD50 of <2000 mg/kg. May be carcinogen/teratogenic.
Applikation / Application:
Selection of stably transformed cells expressing the neomycin resistance gene (aminoglycoside) of transposons Tn 5 or Tn 601.Quelle / Source:
seaweedSicherheits Hinweise / Safety
Klassifizierungen / Classification
Documents:
Safety Data SheetCertificate
Product description
Category List
Genaxxon's G418 is an alternative to ThermoFisher / Invitrogen product no. 10131035 and 10131027, or a replacement for BioChrom product no.: A 2812.
Do not use G418 together wirh anitbiotic/antifungal preparations (e.g. Pen/Strep). These agents are competitive inhibitors of G418. Other antibiotics are potentially cross-reactive as well.
Working concentrations:
Cell type Selection concentration (mg/mL)
HeLa 200 - 600
3T3 cells 500 - 1000
CHO 200 - 400
HEK 293 500 - 800
Jurkat cells 600 - 700
Source: NCBI PubMed
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Nils Hampel 1, Jacqueline Georgy, Mehrnaz Mehrabipour, Alexander Lang, Isabell Lehmkuhl, Jürgen Scheller, Mohammad R. Ahmadian, Doreen M. Floss, Roland P. Piekorz
FEBS Open Bio. 2023 Dec;13(12):2187-2199.
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PMCID: PMC10699113.
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PLoS One. 2020 Apr 1;15(4):e0230804.
doi: 10.1371/journal.pone.0230804.
PMCID: PMC7112226.
Synthetic interleukin 22 (IL-22) signaling reveals biological activity of homodimeric IL-10 receptor 2 and functional cross-talk with the IL-6 receptor gp130.
Sofie Mossner, Marcus Kuchner, Nastaran Fazel Modares, Birgit Knebel, Hadi Al-Hasani, Doreen M Floss, Jürgen Scheller
J Biol Chem. 2020 Aug 28;295(35):12378-12397.
doi: 10.1074/jbc.RA120.013927.
PMCID: PMC7458808.
IL-23R Signaling Plays No Role in Myocardial Infarction
Erika Engelowski, Nastaran Fazel Modares, Simone Gorressen, Pascal Bouvain, Dominik Semmler, Christina Alter, Zhaoping Ding, Ulrich Flögel, Jürgen Schrader, Haifeng Xu, Philipp A. Lang, Jens Fischer, Doreen M. Floss, Jürgen Scheller
Sci Rep. 2018; 8: 17078. Published online 2018 Nov 20. doi: 10.1038/s41598-018-35188-8
PMCID: PMC6244091
Defining the functional binding sites of interleukin 12 receptor β1 and interleukin 23 receptor to Janus kinases
Doreen M. Floss, Tobias Klöcker, Jutta Schröder, Larissa Lamertz, Simone Mrotzek, Birgit Strobl, Heike Hermanns, Jürgen Scheller
Mol Biol Cell. 2016 Jul 15; 27(14): 2301–2316.
doi: 10.1091/mbc.E14-12-1645
PMCID: PMC4945146
MicroRNA-34a promotes genomic instability by a broad suppression of genome maintenance mechanisms downstream of the oncogene KSHV-vGPCR
Claudia J. Krause, Oliver Popp, Nanthakumar Thirunarayanan, Gunnar Dittmar, Martin Lipp, Gerd Müller
Oncotarget. 2016 Mar 1; 7(9): 10414–10432.
doi: 10.18632/oncotarget.7248
PMCID: PMC4891129
Non-Canonical Interleukin 23 Receptor Complex Assembly: P40 PROTEIN RECRUITS INTERLEUKIN 12 RECEPTOR β1 VIA SITE II AND INDUCES P19/INTERLEUKIN 23 RECEPTOR INTERACTION VIA SITE III
Jutta Schröder, Jens M. Moll, Paul Baran, Joachim Grötzinger, Jürgen Scheller, Doreen M. Floss
J Biol Chem. 2015 Jan 2; 290(1): 359–370.
doi: 10.1074/jbc.M114.617597
PMCID: PMC4281739
Alternative Intronic Polyadenylation Generates the Interleukin-6 Trans-signaling Inhibitor sgp130-E10
Jan Sommer, Christoph Garbers, Janina Wolf, Ahmad Trad, Jens M. Moll, Markus Sack, Rainer Fischer, Joachim Grötzinger, Georg H. Waetzig, Doreen M. Floss, Jürgen Scheller
J Biol Chem. 2014 Aug 8; 289(32): 22140–22150.
doi: 10.1074/jbc.M114.560938
PMCID: PMC4139227
