DMEM with L-Glutamine, with 4.5g/L Glucose, w/o Phenol red
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Product information "DMEM with L-Glutamine, with 4.5g/L Glucose, w/o Phenol red"
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
DMEM contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. It was originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels (4.5g/L) for cells with a high energy demand.
DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). As DMEM uses a sodium bicarbonate buffer system (3.7g/L), a 5–10% CO2 environment is necessary to maintain physiological pH.
Genaxxon offers a variety of DMEM modifications for a range of cell culture applications. If you can't find the medium you are looking for we will be glad to prepare the requested medium you do need. Just let us know your needs: info@genaxxon.com.
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Documents:
ProtocolsGeneral Data 1
Source: NCBI PubMed
