Glasgow-MEM (BHK 21) powder w/o L-Glutamine, w/o Tryptose phosphate
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Product information "Glasgow-MEM (BHK 21) powder w/o L-Glutamine, w/o Tryptose phosphate"
Glasgow's Minimum Essential Medium (GMEM) was originally developed by Ian McPherson and Michael Stoker as a modification of Eagle's medium. It was utilized to study the genetic factors which affected cell competence. Glasgow's MEM was developed for use with kidney cells lines, such as BHK-21.
Glasgow's MEM is unique from other media as it contains twice the concentration of amino acids and vitamins than the original Basal Medium Eagle, and is used without serum. Glasgow's MEM was originally formulated with 10% tryptose phosphate broth.
Glasgow's MEM contains no proteins, lipids, or growth factors. Therefore, Glasgow's MEM requires supplementation, commonly with 10% tryptose phosphate broth. Glasgow's MEM uses a sodium bicarbonate buffer system (2.75g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Genaxxon offers a variety of cell culture media of different modifications for a large range of cell culture applications with a focus on custom made media where our customers can choose the incredients according to their needs. Just ask: info@genaxxon.com.
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Documents:
ProtocolsGeneral Data 1
Source: NCBI PubMed