DAPI - 4',6-Diamidino-2-phenylindole dihydrochloride, min. 98%
Advantages at a glance
- cell permeable fluorescent dye
- binds selectively to DNA
- binds to the minor groove of double-stranded DNA
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Product information "DAPI - 4',6-Diamidino-2-phenylindole dihydrochloride, min. 98%"
4',6-Diamino-2-phenylindole hydrochloride is a fluorescent dye binding selectively to the minor groove of double strand DNA (preferentially to AT rich DNA). Forms a stable complex which fluoresces approximately 20 times more than DAPI alone.
DAPI, once added to tissue culture cells, is rapidly taken up into cellular DNA, yielding highly fluorescent nuclei and no detectable cytoplasmic fluorescence.
When the cells are contaminated with Mycoplasmas, characteristic discrete fluorescent foci are readily detected over the cytoplasm and sometimes in intercellular spaces. Also used for flourescent chromosome staining or to stain brain stem cells.
Preparation of solutions:
Stock solution: Dissolve in distilled water to a final concentration of 1 to 5mg/mL. NOTE: Do not use any buffers. The stock solution is stable at -15°C to -25°C for about 12 months.
Working solution: Dilute the stock solution with methanol to a final concentration of 1μg/mL. The working solution is stable at +2 to +8°C for about 6 months.
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