Orange G (C.I. 16230)
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M3180.0010
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Product information "Orange G (C.I. 16230)"
Orange G (C.I. 16230) can be used as tracking dye for gel electrophoresis.
To stop the electrophoretic mobility-shift assay, 5μL of a 10% glycerol / 0.01% orange G mixture is added to 20μL sample volume. The samples were then applied directly to a 5% polyacrylamide gel (40: 1). In agarose gel electrophoresis, Orange G is used as a tracking dye. Since Orange G "runs" at about 20bp to 40bp, it does not cover DNA bands in the range larger than 60bp.
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