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SafeGel green stain for DNA electrophoresis

Genaxxon bioscience
Comparison of emission spectra safegel green stain and safegel red stain

Advantages at a glance

  • Safer than EtBr
  • Easy disposal 
  • Ultra-sensitive: Much more sensitive than EtBr with much lower background
  • Extremely stable
  • Perfectly compatible with a standard UV transilluminator 

€275.00*

Prices excl. VAT plus shipping costs

Volume
Product number: M3191.2000
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.

Shipment: not cooled. Store at +15°C to +30°C. For laboratory usage only!
Product information "SafeGel green stain for DNA electrophoresis"

SafeGel green stain is an ultra sensitive, extremely stable fluorescent dye designed to replace the toxic and possibly mutagenic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. SafeGel green stain is far more sensitive than EtBr without requiring a destaining step. SafeGel green stain is far less toxic and mutagenic compared to EtBr while both show virtually the same UV-spectra, so you can directly replace EtBr with SafeGel green stain without changing your existing imaging system.

SafeGel can be used to stain dsDNA, ssDNA or RNA in agarose gel via either precast or post gel staining. SafeGel can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining. SafeGel is also compatible with downstream DNA manipulations such as digestion with a restriction enzyme, Southern blotting techniques and clonings. A series of safety tests has confirmed that SafeGel is noncytotoxic, nonmutagenic and nonhazardous even at concentrations above the working concentrations used in gel staining. As a result, SafeGel can be safely disposed in regular trash, providing convenience and reducing cost in waste disposal.

This fluorescent dye is supplied as a 10,000X solution in water. For customers who look for large pack size, we offer a cost-saving bulk pack size of 2mL, 5mL or 10mL (M3193.1010).

For high-class agarose gels we offer the Genaxxon standard agarose LE > or our speciality agaroses for high resolution Tiny (M3046) > and Tiny HT (M3047) >.

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FAQs
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Yes, you can use the same filters and camera settings of your imaging system for SafeGel as for gels with EtBr. Please note the emission spectra of SafeGel for specific wavelengths. SafeGel and EtBr have virtually the same emmision spectrum, so you can replace EtBr directly with SafeGel without having to change your existing imaging system.
SafeGel is far more sensitive than EB without requiring a destaining step.
SafeGel is available as a 10000X aqueous solution. It is stable at room temperature (+15 °C to +30 °C) for long-term storage. It can be microwaved but this is not recommended.
Since SafeGel is non-toxic, non-mutagenic and non-hazardous when used at the recommended working concentration, it can be easily disposed of with normal waste. Separate handling of the waste as with EtBr is therefore not necessary. If necessary, obtain information from your local waste disposal company.
SafeGel should be stored at room temperature (+15 °C to +30 °C) protected from light.
SafeGel is used for pre- and post-staining of agarose gels. We recommend using SafeGel in an in-slot application for best results.
Yes, SafeGel can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gels in the post-staining protocol.
Yes, SafeGel can be used for formaldehyde gels for staining RNA in the precast-staining protocol. For DGGE, EMSA and PFGE gels we recommend the post-staining protocol.
Yes, an additional loading buffer is needed. We routinely use 6X loading buffer containing 15% glycerol, 7.5% Ficoll 400, 0.05% Bromophenol Blue. In internal testing 6X loading buffer containing 0.1% Orange G produced good results in precast and post-stained gels. SDS in loading buffer may contribute to band smearing in precast SafeGel gels. If this occurs, we recommend using the post-staining protocol.
No, you can use the dye in room light; however, we recommend storing the dye in the dark.
We recommend in-slot application with a 20X SafeGel red stain sample mix.
Some users have reported being able to detect less than 0.1ng DNA. However, the limit of detection will depend on instrument capability and exposure settings.
SafeGel red stain most likely binds by a combination of intercalation and electrostatic interaction.
The chemical structure of SafeGel red stain is proprietary.
Yes, SafeGel red stain may be removed from DNA by phenol/chloroform extraction and ethanol precipitation.
Yes, SafeGel red stain is compatible with downstream DNA applications such as cloning, ligation and sequencing. We recommend gel extraction kits, Exo-Sap protocol or phenol-chloroform extraction to remove the dye from DNA. Some users have reported performing PCR on DNA in the presence of SafeGel red stain with no purification step.
Yes, customer have reported using SafeGel red stain in cesium gradients. To extract SafeGel red stain from DNA after cesium banding, we recommend adding SDS to a final concentration of 0.1% before butanol extraction. Alternatively, chloroform can be used instead of butanol for extraction.
Yes, SafeGel red stain can be used for blotting. We recommend using the post-staining protocol for blotting applications.
Yes, it is possible, but we do not recommend reusing SafeGel red stain pre-cast gels as signal decreases with subsequent electrophoresis.
Dye precipitation may occur at lower temperatures (winter times), resulting in lower signal or the appearance of precipitate on the surface of the gel. If this occurs, heat the solution to 45-50°C for two minutes and vortex.
While we recommend that you protect the dye from light during long term storage, we have had customers report using SafeGel red stain with success after accidentally leaving it in ambient light for more than one month.
  1. Reduce the amount of DNA loaded by one-half to one-third. Blown out or smeared bands can be caused by overloading. This is frequently observed with DNA ladders.
  2. Perform post-staining instead of pre-casting.
  3. Pour a lower percentage agarose gel for better resolution of large fragments.
  4. Change the running buffer. TBE buffer has a higher buffering capacity than TAE.
  5. Loading buffers containing SDS may contribute to band smearing. If this occurs, use the post-staining protocol for applications requiring SDS-containing loading buffers.
SafeGel is designed to be larger than other dyes to prevent it from entering cells, thus rendering the dye safer. The migration of DNA may be affected depending on the dye:DNA ratio.

  1. Reduce the amount of DNA loaded by on-half to one-third.
  2. Reduce the amount of dye used, i.e., use 0.5X in pre-cast gels.
  3. Post-stain the gel in 3X SafeGel to avoid any interference the dye may have on migration during electrophoresis.
The dye may have precipitated out of solution.
  1. Heat SafeGel solution to 45-50°C for two minutes and vortex to redissolve. 
  2. Store dye at room temperature to avoid precipitation.

Specifications:
10000 times concentrated solution in water.
Blue/Green excitation at 480nm to 530nm but also useable
with a standard transilluminator (254nm to 366nm)

SafeGel can also be used to stain dsDNA, ssDNA or RNA however SafeGel is twice as sensitive to dsDNA than ssDNA or RNA.


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Sicherheits Hinweise / Safety


Klassifizierungen / Classification

eclass-Nr: 32-16-04-03
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.


Documents:

Manuals
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed


How to save money using SafeGel from Genaxxon as DNA staining dye for gel electrophoresis
May 24, 2023 Science
Everything seemed to be easier in the past. At least that’s true when it comes to staining DNA in agarose gels.
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