SafeGel red stain for DNA electrophoresis
Advantages at a glance
- Safer than EtBr
- Easy disposal
- Ultra-sensitive: Much more sensitive than EtBr with much lower background.
- Extremely stable
- Perfectly compatible with a standard UV transilluminator
Ready to ship today,
Delivery time 1-2 workdays
Shipment: not cooled. Store at +15°C to +30°C. For laboratory usage only!
SafeGel red stain is an ultra sensitive, extremely stable fluorescent dye designed to replace the toxic and possibly mutagenic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. SafeGel red stain is far more sensitive than EtBr without requiring a destaining step. SafeGel red stain is far less toxic and mutagenic compared to EtBr while both show virtually the same UV-spectra, so you can directly replace EtBr with SafeGel red stain without changing your existing imaging system.
SafeGel can be used to stain dsDNA, ssDNA or RNA in agarose gel via either precast or post gel staining. SafeGel can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining. SafeGel is also compatible with downstream DNA manipulations such as digestion with a restriction enzyme, Southern blotting techniques and clonings. A series of safety tests has confirmed that SafeGel is noncytotoxic, nonmutagenic and nonhazardous even at concentrations above the working concentrations used in gel staining. As a result, SafeGel can be safely disposed in regular trash, providing convenience and reducing cost in waste disposal.
This fluorescent dye is supplied as a 10,000X solution in water. For customers who look for large pack size, we offer a cost-saving bulk pack size of 2mL, 5mL or 10mL (M3193.1010).
Read in our blog why you should use Genaxxon's SafeGel now.
For high-class agarose gels we offer the Genaxxon standard agarose LE > or our speciality agaroses for high resolution Tiny (M3046) > and Tiny HT (M3047) >.
- Reduce the amount of DNA loaded by one-half to one-third. Blown out or smeared bands can be caused by overloading. This is frequently observed with DNA ladders.
- Perform post-staining instead of pre-casting.
- Pour a lower percentage agarose gel for better resolution of large fragments.
- Change the running buffer. TBE buffer has a higher buffering capacity than TAE.
- Loading buffers containing SDS may contribute to band smearing. If this occurs, use the post-staining protocol for applications requiring SDS-containing loading buffers.
- Reduce the amount of DNA loaded by on-half to one-third.
- Reduce the amount of dye used, i.e., use 0.5X in pre-cast gels.
- Post-stain the gel in 3X SafeGel to avoid any interference the dye may have on migration during electrophoresis.
- Heat SafeGel solution to 45-50°C for two minutes and vortex to redissolve.
- Store dye at room temperature to avoid precipitation.
Specifications:
10000 times concentrated solution in water.
can be used with a standard transilluminator (254nm to 366nm)
no changes in optical settings compared to Ethidium bromide
SafeGel can also be used to stain dsDNA, ssDNA or RNA however SafeGel is twice as sensitive to dsDNA than ssDNA or RNA.
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-04-03
Documents:
Safety Data SheetProtocols
Manuals
General Data 2
Source: NCBI PubMed
Tips for SafeGel red stain applications (gels: 1.5% agarose)
We recommend the in-slot application of a 20-fold SafeGel red stain sample mixture
1. in-slot application:
Dilution of the SafeGel red stain stock solution:
3μL SafeGel red stain (10 000X) is diluted with 97μL H2O or 6X loading buffer. This results in a 300X SafeGel red stain solution.
8µL of the 300X SafeGel red stain solution is diluted in 112µL of 6X loading buffer. This gives the 20X SafeGel red stain working solution.
Add 1µL to 2µL of the working solution to 4µL of sample (PCR preparation) and apply directly to the slot of the agarose gel.
For even filling of the gel pockets, more 6X loading buffer can be added to ensure even filling of the pockets. The actual quantity depends on the user's individual experience and should be adjusted individually.
2. SafeGel red stain in the gel (Precast):
In 100mL TBE/ TAE gel, 10μL SafeGel red stain is recommended, it is possible to reduce down to 3.5µL SafeGel red stain / 100mL gel (it must be tested whether the intensity of the DNA bands meets the user's needs).
3. poststaining:
3X staining solution in water with 0.1M NaCl (increases sensitivity):15μL SafeGel red stain stock solution and 5mL NaCl to 45mL water
Leave the gel in the staining bath for approx. 30 min, normal UV illustration.
4. SafeGel red stain for Southern blot:
It is possible to detect the transfer of DNA to a membrane with a SafeGel red stain stained agarose gel. This does not interfere with subsequent steps.Blot according to normal electroblotting protocol. Check the blotting membrane under UV light.
Proceed as usual. No decolorization necessary.
