4,5-diaminofluorescein diacetate - DAF-2 DA

Genaxxon bioscience

4,5-Diaminofluoresceindiacetat - DAF-2 DA

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Weight

1 mg

5 mg

Product number: S5440.0001
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.

Shipment: not cooled. Store at -20°C. For laboratory usage only!

Product information "4,5-diaminofluorescein diacetate - DAF-2 DA"

4,5-diaminofluorescein diacetate (DAF-2 DA) is a highly sensitive probe for the real time detection of NO in vivo. Cell permeable.

Application: DAF-2 DA is probably the most successful indicator for nitric oxide. As with other fluorescein diacetates, DAF-2 diacetate is membrane permeant and is deacetylated by intracellular esterases to 4,5-diaminofluorescein (DAF-2). Can be used for the fluorimetric detection of nitric oxide and in fluorescence microscopy to measure realtime changes in nitric oxide levels in vivo.

References:
V.M. Dirsch et al.; Biol. Proc. Online 5, 136 (2003)
N. Nagata et al.; J. Biochem. 125(4), 658 (1999)
H. Kojima et al.; Anal. Chem. 70(13), 2446 (1998)
H. Kojima et al.; Chem. Pharm. Bull. 46, 373 (1998)
H. Kojima et al.; Neuroreport 9, 3345 (1998)

Specifications:
Purity: >98% (HPLC)
Appearance: Off-white powder
Identity: determined by NMR
C24H18N2O7
MW = 446.41 g/mol
Fluorescence: λex 491 nm; λem 513 nm
Solubility: soluble in DMSO

Applikation / Application:

fluorimetric detection of nitric oxide and in fluorescence microscopy to measure real-time changes in nitric oxide levels in vivo.

Einheiten / Units:

Quelle / Source:

synthetic

Sicherheits Hinweise / Safety

H-Sätze: H302

Klassifizierungen / Classification

CAS-Nr: 205391-02-2
eclass-Nr: 30-19-60-90

Documents - Protocols - Downloads :

Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

Documents:

Safety Data Sheet
Certificate
General Data 1
Category List

Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed

The Hemoglobin Bjgb From Bradyrhizobium diazoefficiens Controls NO Homeostasis in Soybean Nodules to Protect Symbiotic Nitrogen Fixation.
Ana Salas, Germán Tortosa, Alba Hidalgo-García, Antonio Delgado, Eulogio J Bedmar, David J Richardson, Andrew J Gates, María J Delgado
Front Microbiol. 2020 Jan 10;10:2915.
doi: 10.3389/fmicb.2019.02915.
PMCID: PMC6965051.

C-Terminal Tyrosine Residue Modifications Modulate the Protective Phosphorylation of Serine 129 of α-Synuclein in a Yeast Model of Parkinson's Disease.
Alexandra Kleinknecht, Blagovesta Popova, Diana F Lázaro, Raquel Pinho, Oliver Valerius, Tiago F Outeiro, Gerhard H Braus
PLoS Genet. 2016 Jun 24;12(6):e1006098.
doi: 10.1371/journal.pgen.1006098.
PMCID: PMC4920419.