5-FAM, single isomer
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Product information "5-FAM, single isomer"
The single isomer 6-FAM (6-Carboxyfluorescein) is an amine-reactive derivative of Fluorescein giving stable derivatives upon conjugation with biopolymers. The single amine-reactive fluorescein derivatives 5-FAM > and 6-FAM > and the mixed isomers form 5/6-FAM > are the most common fluorescent derivatizing reagents for the covalently labeling of proteins.
Carboxyfluorescein can be retained much better in cells than fluorescein since its pKa is lower than the cytosolic pH of most cells.
5-FAM, 6-FAM and 5/6-FAM contain a carboxylic acid which can react with primary amines via carbodiimide activation. Carboxyfluorescein is commonly used as a polar tracer.
Other fluorescent dyes from Genaxxon: TAMRA >, ROX >, DAF-2 >
Specifications:
Purity: ≥99% (Titr.)
Identity: Determined by NMR/IR
Appearance: orange to yellow orange powder
Soluble in: DMSO
MW = 376.32 g/mol
Fluorescence: λex 492 nm; λem 517 nm in 0.1M Tris pH8.0
Applikation / Application:
fluorescent dye for labelling of proteinsQuelle / Source:
syntheticKlassifizierungen / Classification
Documents:
CertificateGeneral Data 1
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