5-ROX, single isomer
Advantages at a glance
- Purity: >95% (HPLC)
- Appearance: dark violet powder
- Identity: by NMR/IR
- λex 575nm; λem ~600nm in 0.1M Tris pH8.0 (ε = 92000 M-1cm-1)
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Product information "5-ROX, single isomer"
5-ROX (5-carboxy-X-rhodamine) is a derivative of the amine-reactive carboxy-X-rhodamine (ROX). Compared to the fluoresceine family of dyes, Rhodamine dyes are more resistant to photo bleaching and stable over a wider pH range. The relatively long wavelength emission maximum for ROX makes it an attractive choice in multiplex detection systems. 5-ROX >, 6-ROX > and their mixture 5/6-ROX > are used in the labeling of biomolecules by EDC-mediated reactions. Derivatives of ROX are used in the labeling of proteins, peptides and other biological ligands. Succinimidyl ester with the derivative 5/6-carboxy-X-rhodamine (5(6) Carboxy-X-rhodaminsuccinimidylester / 5(6) ROX SE >) are frequently used in the oligonucleotide labeling and for automated applications in DNA sequencing.
Genaxxon bioscience offers a wide range of fluorescent dyes for designing your probe or for other research purposes. More fluorescent dyes > from Genaxxon: S5409 FAM >, S5410 TAMRA >, S5439 DAF-2 >, etc.
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