DMSO, Molecular biology grade
Advantages at a glance
- Purity: min. 99.5% (titr.)
- DNases, RNases: not detectable
- Density (d20°/4°): 1.100
- Free acid: max: 0.001%
- Water (K.F.): max. 0.1%
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Product information "DMSO, Molecular biology grade"
DMSO is an aprotic polar solvent that is used in chemical reactions as well as in PCR or as anti-freeze protection in the deep-freeze storage of cells, tissues and organs (sterile DMSO only).
By adding DMSO for annealing before the sequencing reaction and adding 10% DMSO (final concentration) for the labeling reaction and for termination, the quality of the sequencing of Miniprep DNA can be increased. The amplification of RNA by RT-PCR is also improved by adding DMSO. DMSO is used during reverse transcription (RT) and PCR. The DMSO concentration depends on the RNA species.
Specifications:
Purity: min. 99.5% (titr.)
DNases, RNases: not detectable
Density (d20°/4°): 1.100
Free acid: max: 0.001%
Non-volatile matter: max. 0.001%
Water (K.F.): max. 0.1%
MP = 18°C
BP = 189°C
C2H6OS
MW = 78.1 g/mol
Applikation / Application:
- DMSO for improved annealing before the sequencing reaction by adding 10% DMSO (final concentration) for the labeling reaction and for termination. - Improved amplification of RNA by RT-PCR by adding DMSO. - For improved reverse transcription (RT) and PCR. The DMSO concentration depends on the RNA species. - For freezing cells but only after sterile filtration of product M6324.Klassifizierungen / Classification
Documents:
Safety Data SheetCertificate
Source: NCBI PubMed
