qPCR adhesive plate seals

Advantages at a glance

Pressure activated adhesive
Peelable
Optically clear

€156.28

pack size

50 seals

100 seals

Product number: I2248.0050

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Delivery time: 3-8 days

Product information "qPCR adhesive plate seals"

The Genaxxon qPCR adhesive seals are made of a optically clear adhesive film, with a pressure activated clue. The film is peelable and thus perfectly suitable for qPCR and other imaging techniques including crystallization applications. The adhesive is released after pressure on the surface.

- Optically clear seal specifically developed for optical applications, particularly qPCR
- It is non sticky when removed from the packaging; this aids handling when wearing gloves
- The adhesive is contained within small capsules, allowing light to pass through to ensure the optical clarity of the seal
- Seal integrity range: -40 °C to 110 °C
- Free from DNase, RNase, and human genomic DNA

When the seal is in position, pressure can be applied to burst the capsules, releasing a strong adhesive only where the seal touches the raised well rims of the plate - the rest of the seal area above the wells remains optically clear

FAQs

Yes, the used plates and foils could influence the assay performance according to several scientific studies (e.g. Reiter and Pfaffl 2008: Effects of Plate Position, Plate Type and Sealing Systems on Real-Time PCR Results, Biotechnology & Biotechnological Equipment, 22:3, 824-828, DOI: 10.1080/13102818.2008.10817561).

In general, white qPCR plates (e.g. Genaxxon’s I2017) have a lower light scattering and thus a higher signal strength than transparent qPCR plates (e.g. Genaxxon’s I2004, I2010, I2031). If you have a weakly expressed target or a only small amounts of sample material, we recommend using a white qPCR plate.

Beside this, it is essential to use optically clear foils for your qPCR. We do not recommend using “normal” PCR foils as adhesive residues and transparency differences in the material may lead to inhomogeneity during your qPCR assay.

Genaxxon offers different qPCR foils: a strongly adhesive foil (I2012) that securely seal the plate immediately, and a pressure-sensitive foil (I2248) that adheres to the plate by applying strong pressure.

Documents - Protocols - Downloads :

Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

Documents:

Spec. Product Description

Replacement for: ABI® MicroAmp Optical Adhesive film, Absolute qPCR plate seals and Roche LightCycler® sealing foils