Nucleic acid Extraction Solution

The non-toxic DNA Q-Extraction Solution is optimized for easy extraction of PCR-ready DNA. DNA is easily extracted from various mammalian tissues e.g. mouse tails, ear snips, liver, kidney, lung and saliva.

DNA Q-Extraction Solution provides fast and easy extraction of nucleic acids from various mammalian tissues (e.g. mouse tails, ear snips, liver, kidney, lung), saliva and bacteria. The PCR-ready nucleic acid is extracted, using one tube and one reaction, in as little as 8 minutes.

The one-step lysis is performed in either a thermocycler or heating block and is divided into two simple heating steps. The extracted DNA is then ready for PCR without further handling such as vortex, centrifugation or dilutions. The obtained DNA extract is stable at - 20°C for up to one week and at - 80°C for long term storage.

For optimal genotyping results we recommend to use our DNA Q-Extraction Solution together with our Red Mastermix Master Mix (2X) for PCR (M3029 >). E.g. use 2µL to 5µL of the Q-Extract solution for a 25µL PCR reaction. Protocol details: see document section. 

Features:
- One-reagent set-up
- Minimal handling
- Rapid 8-minute protocol
- PCR-ready DNA
- DNA extracts from mammalian tissues and bacteria
- Non-toxic reagents
- Scalable set-up
- Automation-friendly

Extraction Protocol
Preparation of DNA extraction should be performed in a separate area from that used for setting up the PCR reaction.

1. Thaw DNA Q-Extraction Solution.
For the first time use, aliquot the Q-Extract DNA Extraction solution into smaller volumes. (DNA Q-Extraction Solution has a cloudy appearance).
2. Add your sample to a tube containing 100µL DNA Q-Extraction Solution. Recommended sample sizes are shown in Table 1 of the data sheet.
3. Vortex the tube containing the sample and the DNA extraction solution for 15 sec. Make sure that the sample is completely covered by the DNA Q-Extraction Solution.
4. Transfer the tube to a heat block or a thermal cycler and incubate for

1. 65 °C for 6 min
2. 98 °C for 2 min
3. 4 °C (or cool down on ice)

The DNA extract is now ready for PCR.

Please check also our DNA purification kits >.