G418 - 50mg/mL
Order number: M3118.0020
Shipping: Shipment: not cooled. Store at +2°C to +8°C. For laboratory usage only!CAS-Nr: 108321-42-2
Delivery time: 3- 8 working days
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G418 also known as Geneticin(TM), is an aminoglycoside antibiotic that inhibits protein synthesis in mammalian cells by interfering with ribosomal function. G418 shows a structure similar to gentamycin > and kanamycin > and finds application in the selection of stably transformed cells expressing the neomycin resistance gene (aminoglycoside) of transposons Tn 5 or Tn 601.
Because G418 is toxic for many cells, the optimum concentration for each cell type has to be determined. It is usually between 50ug/mL and 1000μg/mL. At a concentration of 500μg/mL, for example, 70% of HepG2 cells are killed after incubation for one week and 100% will stop their growth. Since potency of the antibiotic can vary from batch to batch, it is recommended to create large stocks of a batch tested to standardize the system.
Cells divide still once or twice in the presence of the lethal dose of G418, so that a selection effect will be visible only after several days. Geneticin is a registered trademark of Gibco, a Division of Life Technologies.
Genaxxon's G418 is an alternative to ThermoFisher / Invitrogen product no. 10131035 and 10131027, or a replacement for BioChrom product no.: A 2812.
Genaxxon bioscience offers a broad range of Antibiotics and Antimycotics as powder or solutions.
G418 inhibits protein synthesis in mammalian cells by interfering with ribosomal function.
Technical Data:
Specifications:
50 mg/mL sterile filtered solution
C20H40N4O10x2H2SO4
MW = 692.7 g/mol
Liquid formulation of Cat#: M3117
Harmful: LD50 of <2000 mg/kg. May be carcinogen/teratogenic.
application:
Selection of stably transformed cells expressing the neomycin resistance gene (aminoglycoside) of transposons Tn 5 or Tn 601.Source
seaweedSicherheits Hinweise / Safety
H-Sätze: H301R-Sätze: R61
Gefahrstoffkürzel: T
Klassifizierungen / Classification
CAS-Nr: 108321-42-2eclass-Nr: 32-19-90-90
Dokumente - Protokolle - Downloads
Here you will find information and further literature on G418 - 50mg/mL. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.
Dokumente - Protokolle - Downloads
Genaxxon's G418 is an alternative to ThermoFisher / Invitrogen product no. 10131035 and 10131027, or a replacement for BioChrom product no.: A 2812.
Do not use G418 together wirh anitbiotic/antifungal preparations (e.g. Pen/Strep). These agents are competitive inhibitors of G418. Other antibiotics are potentially cross-reactive as well.
Working concentrations:
Cell type Selection concentration (mg/mL)
HeLa 200 - 600
3T3 cells 500 - 1000
CHO 200 - 400
HEK 293 500 - 800
Jurkat cells 600 - 700
Referenzen..
Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
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