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Agarose LM - low melting

Advantages at a glance

  • Low melting agarose
  • ß-Agarase digestion possible
  • For analytical gels
  • For preparative gels


% €145.00* (20% saved)
Product number: M3049.0025
Ready to ship today,
Delivery time 1-2 workdays

Shipment: not cooled. Store at +15°C to +30°C. For laboratory usage only!
Product information "Agarose LM - low melting"

Agarose LM is the original low melting agarose. This "molecular biology grade" agarose gives gels with better properties and higher transparency than the standard normal melting point agarose. The agarose is equivalent to SeaPlaque™ from Lonza. Agarose LM is a low melting agarose with a very high separation capacity for large DNA fragments (>1000 bp), RNA and proteins. Enzymatic manipulations (eg, digestion, ligation, PCR, etc.) can be performed in the molten gel. Extraction of the DNA is therefore not necessary. Low melting Agarose LM is excellently suited for digestion with β-agarase (S5223). Large DNA fragments can be isolated very gently. The low melting agarose LM is used in the analytical and preparative field. For optimal results, the gel should be stored for at least 1 hour before use at + 2 ° C to + 8 ° C.

Genaxxon offers agaroses for a wide variety of applications. There are agaroses with different melting points or with normal or high-resolution separation properties of DNA fragments.

Standard melting point agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 50bp up to 20kbp. This agarose is available as powder (Standard Agarose LE (M3044 >)) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. These are compact, pre-weight and therefore easy-to-use.

Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, Agarase (S5223) >, as well as for “in-gel’ DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel.

High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®).

For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.

Electroendosmosis (EEO): <0.10
Gel strength (1.0%): >200 g/cm2
Gel strength (1.5%): >1000 g/cm2
Gelling temperature (1.5%): <30°C
Melting temperature (1.5%): <65°C
Loss on drying: max. 10%
DNAse and RNAse: not detected

Applikation / Application:
- for electrophoresis of DNA, RNA and proteins. - for enzymatic reaction in liquid agarose (in gel applications) - for immobilization of micro organisms or virus plaque assays

Einheiten / Units:
Quelle / Source:

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

EC-Nr: 232-731-8
CAS-Nr: 9012-36-6
eclass-Nr: 32-16-04-03
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.


Safety Data Sheet
Spec. Product Description
General Data 1
General Data 2
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed

Quelle/Source: NCBI PubMed >

An advanced glioma cell invasion assay based on organotypic brain slice cultures

Tanja Eisemann, Barbara Costa, Jens Strelau, Michel Mittelbronn, Peter Angel, Heike Peterziel

BMC Cancer. 2018; 18: 103. Published online 2018 Jan 30. doi: 10.1186/s12885-018-4007-4

PMCID: PMC5789681