Agarose LM - low melting

Genaxxon bioscience

Advantages at a glance

Low melting agarose
ß-Agarase digestion possible
For analytical gels
For preparative gels

€116.00*

Prices excl. VAT plus shipping costs

Weight

10 g

25 g

100 g

Product number: M3049.0025
Ready to ship today,
Delivery time 1-2 workdays

Shipment: not cooled. Store at +15°C to +30°C. For laboratory usage only!

Product information "Agarose LM - low melting"

Agarose LM is the original low melting agarose. This "molecular biology grade" agarose gives gels with better properties and higher transparency than the standard normal melting point agarose. The agarose is equivalent to SeaPlaque™ from Lonza. Agarose LM is a low melting agarose with a very high separation capacity for large DNA fragments (>1000 bp), RNA and proteins. Enzymatic manipulations (eg, digestion, ligation, PCR, etc.) can be performed in the molten gel. Extraction of the DNA is therefore not necessary. Low melting Agarose LM is excellently suited for digestion with β-agarase (S5223). Large DNA fragments can be isolated very gently. The low melting agarose LM is used in the analytical and preparative field. For optimal results, the gel should be stored for at least 1 hour before use at + 2 ° C to + 8 ° C.

Genaxxon offers agaroses for a wide variety of applications. There are agaroses with different melting points or with normal or high-resolution separation properties of DNA fragments.

Standard melting point agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 50bp up to 20kbp. This agarose is available as powder (Standard Agarose LE (M3044 >)) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. These are compact, pre-weight and therefore easy-to-use.

Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, Agarase (S5223) >, as well as for “in-gel’ DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel.

High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®).

For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.

FAQs

The pore size of the agarose gel depends on the concentration of the agarose. Higher concentrations lead to smaller pores and a firmer gel, which affects the separation of larger DNA fragments. Lower concentrations result in a softer gel with larger pores for smaller fragments.

The choice of the right agarose depends on the size of the DNA or RNA fragments you want to separate, as well as the type of application planned. Genaxxon offers a range of agaroses, including standard agarose, high-resolution agarose, and low-melting agarose, which are suitable for various applications such as blotting, PFGE, in-gel analyses, and more. If you're unsure which agarose is right for your application, feel free to contact our technical support at info@genaxxon.com.

For separating small DNA fragments or for high-resolution applications, high-resolution agaroses are ideal. These agaroses can reveal differences of just a few base pairs (2-3 bp) and are excellent for allele sizing experiments or mutation analysis. Genaxxon offers two high-resolution agaroses: M3046 – Agarose Tiny and M3047 – Agarose Tiny HT.

Low-melting agarose has a lower melting point and is especially suited for rapid DNA extraction from gels and in-gel processing. It is also ideal for re-isolating DNA and RNA and for conducting phage plaque assays. Genaxxon offers two low-melting agaroses: M3046 – Agarose Tiny and M3049 – Agarose LM – low melting.

Low-melting agarose is particularly useful in cell culture applications, such as phage plaque assays. It aids in the rapid extraction of DNA and is used in cell culture to test virus concentrations. For these applications, Genaxxon offers M3049 – Agarose LM – low melting.

For blotting experiments, such as Southern blotting, Genaxxon recommends agaroses like Agarose LE (M3044), Agarose Tiny HT (M3047), and Agarose Mega (M3051), which enable precise separation and transfer of DNA onto a membrane.

Pulsed-Field Gel Electrophoresis (PFGE) is a method for separating DNA fragments larger than 30 kb. Genaxxon recommends Agarose Mega (M3051) for PFGE, which provides better resolution of large DNA fragments.

Specifications:
Electroendosmosis (EEO): <0.10
Gel strength (1.0%): >200 g/cm2
Gel strength (1.5%): >1000 g/cm2
Gelling temperature (1.5%): <30°C
Melting temperature (1.5%): <65°C
Loss on drying: max. 10%
DNAse and RNAse: not detected

Applikation / Application:

- for electrophoresis of DNA, RNA and proteins. - for enzymatic reaction in liquid agarose (in gel applications) - for immobilization of micro organisms or virus plaque assays

Einheiten / Units:

Quelle / Source:

seaweed

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

EC-Nr: 232-731-8
CAS-Nr: 9012-36-6
eclass-Nr: 32-16-04-03

Documents - Protocols - Downloads :

Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

Documents:

Safety Data Sheet
Certificate
Spec. Product Description
General Data 1
General Data 2

Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed

An advanced glioma cell invasion assay based on organotypic brain slice cultures
Tanja Eisemann, Barbara Costa, Jens Strelau, Michel Mittelbronn, Peter Angel, Heike Peterziel
BMC Cancer. 2018; 18: 103.
doi: 10.1186/s12885-018-4007-4
PMCID: PMC5789681