Agarose Tiny HT high resolution, normal melting
Advantages at a glance
- High resolution agarose
- Separation resolution of 2bp
- Standard melting agarose
Ready to ship today,
Delivery time 1-2 workdays
Shipment: not cooled. Store at +15°C to +30°C. For laboratory usage only!
Our Agarose Tiny HT is a high resolution (+/-2 bp), normal melting agarose for fine resolution of small DNA fragments in the 20 bp to 1500 bp range. It can be used for genotyping, allele sizing and short tandem repeat analysis. Agarose Tiny works as a molecular screen and has twice the resolution of the finest sieving agaroses. It can discriminate between fragments which differ by only 2-4 bp in length and thus, is able to compete with acrylamide gels, being much easier to handle as the latter. Agarose Tiny HT 2% to 4% gels give similar results to Polyacrylamide 6% to 8% gels. For efficient resolution, the concentration of Agarose Tiny HT should be adjusted according to the range of band sizes analyzed, and the gel running buffer used (TAE or TBE).
Genaxxon offers Agaroses for different purposes. Two melting point options and normal or high resolution separation of DNA fragments are available.
Standard agarose high melting point agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 50bp up to 20kbp. This agarose is available as powder (M3044 Agarose LE >) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. These are compact, pre-weight and therefore easy-to-use.
Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, Agarase, as well as for “in-gel’ DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel.
High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®).
For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.
Specification:
Electroendosmosis (EEO): <0.12
Sulfate: <0.10%
Gel strength (1.5%): >2000 g/cm2
Gel strength (3.0%): >4250 g/cm2
Gelling temperature (1.5%): <38°C
Melting temperature (1.5%): <89°C
pH (1% in water): 5 - 7
Loss on drying: max. 7%
Ash: max. 0.7%
DNAse and RNAse: not detected.
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
EC-Nr: 232-731-8CAS-Nr: 9012-36-6
eclass-Nr: 32-16-04-03
Documents:
Safety Data SheetCertificate
Spec. Product Description
General Data 1
General Data 2
Source: NCBI PubMed
Dissection of the complex phenotype in cuticular mutants of Arabidopsis reveals a role of SERRATE as a mediator.
Derry Voisin, Christiane Nawrath, Sergey Kurdyukov, Rochus B Franke, José J Reina-Pinto, Nadia Efremova, Isa Will, Lukas Schreiber, Alexander Yephremov
PLoS Genet. 2009 Oct;5(10):e1000703.
doi: 10.1371/journal.pgen.1000703.
PMCID: PMC2760142.