GenLadder 1 kbp Plus with gel stainining dye
Advantages at a glance
- ready-to-use Formel: enthält nicht nur Ladepuffer, sondern auch Nukleinsäurefärbemittel
- Nicht-toxisches und nicht-mutagenes Gel-Färbemittel
- 13 Fragmente in Größen zwischen 250 - 10.000bp
- Innovation für Hochdurchsatz oder Colony screenings
€51.30
- Free shipping
- Shipping within 24h
- 30 days money back guarantee
- Purchase comfortable on account
Product information "GenLadder 1 kbp Plus with gel stainining dye"
The GenLadder 1kb bp Plus with gel staining dye and loading dye - ready-to-use is an innovation for high throughput approaches or colony screenings! Ideally suited to determine the size of double-stranded DNA between 250 and 10,000 base pairs.
At the same time, the mixture also contains a fluorescent gel staining dye detectable with blue light (ca. 490nm excitation). Additional staining (pre- or post staining) or the addition of fluorescent dye prior to application is thus unnecessary and saves an additional pipetting step.
This DNA ladder is an ideal match for our Red MasterMix Fluoro 2X, which also contains the gel staining dye. Together the ideal pair for Colony Screens!
The Gene Ladder 1kbp Plus consists of 13 fragments in sizes between 250 - 10,000bp.
The 1000bp and 3000bp fragment show stronger intensity to allow easier identification. All fragments are 'blunt-ended'. The GenLadder can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography.
Fragment sizes (in base pairs): 10.000 bp (28ng/6µL), 8000 bp (28ng/6µL), 6000 bp (28ng/6µL), 5000 bp (28ng/6µL), 4000 bp(18ng/6µL), 2x 3000 bp (92ng/6µL), 2500 bp (34ng/6µL), 2000 bp (34ng/6µL), 1500 bp (20ng/6µL), 2x 1000 bp (92ng/6µL), 750 bp (23ng/6µL), 500 bp (30ng/6µL), 250 bp (45ng/6µL).
The DNA marker is already dissolved in buffer containing orange G and Xylene cyanol FF as tracking dyes. The total concentration of the DNA-Marker is 90 µg/mL.
The recommended amount of DNA marker per lane is 0.5-0.6μg (5-6µL).
Please note: The included dye does show a small excitation peak in the UV range, but fluorescence is significantly stronger under blue light excitation. We therefore recommend using blue light to achieve the best possible results.
The GenLadder with gel staining dye is also available as a GenLadder 100bp Plus (M3237).
GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3084 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye.
The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.
