GenLadder 100 bp Plus with loading dye
Advantages at a glance
- ready-to-use loading buffer
- 10 fragments from 100bp to 1000bp
- additional bands at 1500bp and 3000bp
- extra bright bands at 500bp and 1500bp as reference points
- all fragments are blunt-ended
Ready to ship today,
Delivery time 1-2 workdays
Shipment: on wet ice. Store at -20°C. For laboratory usage only!
Our DNA marker GenLadder 100 bp Plus with loading dye is an ideal tool to determine the size of double stranded DNA in the range of 100 - 3000 bp. The additional bands at 1.5kbp and 3.0kbp allow to assign additional DNA fragment sizes in the range up to 3000 bp without using an additional 1kbp DNA marker.
The Gene Ladder 100 bp Plus consists of 12 fragments in sizes between 100 - 1000bp spaced 100bp apart. In addition, there are further bands at 1500bp and 3000bp.
The 500bp and 1500bp fragment show stronger intensity to allow easier identification. All fragments are 'blunt-ended'. The GenLadder can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography.
Fragment sizes (in base pairs): 3000 bp (40ng/5µL), 2x 1500 bp (70ng/5µL), 1000 bp (50ng/5µL), 900 bp (40ng/5µL), 800 bp (40ng/5µL), 700 bp (30ng/5µL), 600 bp (30ng/5µL), 2x 500 bp (90ng/5µL), 400 bp (40ng/5µL), 300 bp (30ng/5µL), 200 bp (40ng/5µL), 100 bp (40ng/5µL).
The DNA marker is already dissolved in buffer containing orange G and Xylene cyanol FF as tracking dyes. The total concentration of the DNA-Marker is 0.1µg/µL (108µg/mL).
The recommended amount of DNA marker per lane is 0,5μg (5µL).
GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye.
The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.
Specifications:
ready-to-use DNA marker containing already loading dye
Number of bands: 12
Fragment sizes and DNA amounts per band:
100 bp (40ng/5µL), 200 bp (40ng/5µL), 300 bp (30ng/5µL), 400 bp (40ng/5µL), 500 bp (2x) (90ng/5µL), 600 bp (30ng/5µL), 700 bp (30ng/5µL), 800 bp (40ng/5µL), 900 bp (40ng/5µL), 1000 bp (50ng/5µL), 1500 bp (2x) (70ng/5µL), 3000 bp (40ng/5µL).
DNA is dissolved in buffer with loading dye at a concentration of 0.1µg/µL (50µg per 500µL).
Buffer contains orange G & xylene cyanol FF as tracking dyes.
We recommend to load 5µL per lane.
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-05-02
Documents:
Safety Data SheetCertificate
Product description
Source: NCBI PubMed
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PMCID: PMC9960570.
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