GenLadder XLarge up to 25 kbp - ready-to-use
Advantages at a glance
- ready-to-use loading buffer
- 14 fragments from 250bp to 25,000bp
- extra bright bands at 1000bp and 3000bp as reference points
- very stable when stored at -20°C
€45.50
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- Shipping within 24h
- 30 days money back guarantee
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Delivery time: 3-8 days
Product information "GenLadder XLarge up to 25 kbp - ready-to-use"
With the synthetic GenLadder XLarge, even larger plasmid insertions and vector plasmids can be determined clearly and precisely in size by gel electrophoresis in the range of 250 - 25000 base pairs after restriction digests. The Genaxxon GenLadder XLarge shows 14 bands. The approximate mass of the DNA in each band is given for a 5µL load (0.52µg/lane). Additionally the 1000 bp and 3000 bp are intensified for better and faster orientation in 1.0 - 1.5% agarose gels.
Please avoid repeated freez-thaw cycles. We recommend to portion the product into smaller aliquots.
Source:
PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10mM Tris-HCl (pH8.0) and 10mM EDTA.
GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye.
The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.
Specifications:
Number of bands: 14
Fragment sizes: 250 bp, 500 bp, 750 bp, 2x 1000 bp, 1500 bp, 2000 bp, 2500 bp, 2x 3000 bp, 4000 bp, 5000 bp, 6000 bp, 8000 bp, 10000 bp, 25000 bp.
DNA is dissolved at a concentration of 0.1µg/µL (52µg per 500µL).
Storage buffer: 10 mM Tris-HCl (pH8.0) and 10mM EDTA with bromophenol blue and xylene cyanol FF as tracking dyes.
We recommend to use 5µL per lane.
Applikation / Application:
Molecular weight standard for DNA electrophoresisQuelle / Source:
syntheticKlassifizierungen / Classification
Documents:
Safety Data SheetProduct description
