DNase I (EC 3.1.21.1) RNase free
Advantages at a glance
- free of RNase
- non-animal origin
€108.35
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Delivery time: 3-8 days
Product information "DNase I (EC 3.1.21.1) RNase free"
Description
RNase-free DNAse I is mainly used to remove DNA from RNA preparations. E.g. for experiments like RT-PCR, RT-qPCR or other applications that should be free of DNA. Since no purification method for RNA removes 100% of the DNA, it is advisable to remove DNA using DNAse I prior to any RT-PCR.
A simple 15-minute digestion at room temperature reliably removes all contaminating DNA.
DNase I is inactivated by adding the stop solution (1µL of a 50mM EDTA solution to 10µL of the DNA digest solution) and subsequent heating. Since heat also denatures RNA, the batch can then be used immediately for reverse transcription.
Our RNase-free DNAse I is a recombinantly derived DNase I. Therefore, there are no problems with possible traces of animal origin.
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