FAST RNA-seq Library Preparation Kit

Advantages at a glance

Broad Compatibility: Suitable for total RNA or mRNA from animals, plants, and fungi.
Flexible Input: Handles RNA input from 10 ng to 1000 ng.
Fast Workflow: Reduces library prep time from 7 hours to 3 hours.
Integrated Process: Combines cDNA synthesis, end repair, and dA-tailing in one step.
Platform Versatility: Works with MGI and Illumina sequencing platforms.

€3,496.50*

pack size

24 reactions

96 reactions

Product number: M4408.0096
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.

Shipment: on dry ice. Store at -20°C. For laboratory usage only!

Product information "FAST RNA-seq Library Preparation Kit"

Genaxxon’s FAST RNA-seq Library Preparation Kit is an all-in-one solution designed to deliver fast and efficient preparation of RNA sequencing libraries. Optimized for use with eukaryotic total RNA or purified mRNA samples from organisms such as animals, plants, and fungi, this kit offers unmatched flexibility with RNA input amounts ranging from 10 to 1000 ng. Whether you're working on high-throughput sequencing projects with MGI or Illumina platforms, this kit ensures high-quality library preparation with minimal effort.

Workflow

Effortlessly prepare your RNA sequencing libraries in less time with an integrated workflow that reduces preparation time from 7 hours to just 3 hours.

Key Features:

Wide Compatibility: Ideal for a variety of eukaryotic samples, including total RNA and purified mRNA from animals, plants, and fungi.
Flexible Input Range: Supports RNA input amounts from as low as 10 ng to as high as 1000 ng, providing versatility for different experimental setups.
Streamlined Efficiency: Combines second-strand cDNA synthesis, dsDNA end repair, and dA-tailing into a single, time-saving step.
Platform Versatility: Fully compatible with MGI and Illumina high-throughput sequencing platforms, ensuring flexibility across various sequencing needs.
Buffer Options: Offers both non-strand-specific and strand-specific buffer options to suit varying experimental requirements.

Streamlined Workflow:

With a three-step process that simplifies second-strand cDNA synthesis, dsDNA end repair, and dA-tailing into one reaction, this kit saves time and minimizes errors associated with intermediate purifications. The workflow is optimized for speed and reliability, ensuring that high-quality libraries are produced efficiently.

Applications:

RNA-seq Library Preparation for MGI and Illumina sequencing platforms
Total RNA and mRNA Library Preparation from a wide range of eukaryotic organisms
Strand-Specific and Non-Strand-Specific RNA Library Preparation, providing flexibility based on experimental needs

Compatibility

This kit is fully compatible with Genaxxon’s Unique Dual Index primer pairs. For 24 reactions, use the UDI MidiSet (M4402). For 96 reactions, you can either use 2x UDI Set A (M4403) or 1x UDI Set A (M4403) and 1x UDI Set B (M4404).

For optimal RNA enrichment, use the Poly (A) mRNA Capture Module with high-quality total RNA samples to avoid introducing a 3' bias during library preparation. For samples with low quality, we recommend using the rRNA Depletion Module to enhance RNA enrichment. In cases where RNA samples from plants or other eukaryotic cells show degradation but still exhibit 28S and 18S bands in agarose gel electrophoresis, increasing the total RNA input and adjusting PCR cycles accordingly can help achieve sufficient library yields.

Specifications:
Suitable for total RNA or purified mRNA with input amounts ranging from 10 ng to 1000 ng.
Compatible with high-throughput sequencing platforms, including Illumina and MGI

Applikation / Application:

RNA-seq Library Preparation for MGI and Illumina sequencing platforms; Total RNA and mRNA Library Preparation from a wide range of eukaryotic organisms; Strand-Specific and Non-Strand-Specific RNA Library Preparation, providing flexibility based on experimental needs

Einheiten / Units:

Quelle / Source:

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 32-16-05-90

Documents - Protocols - Downloads :

Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

Documents:

Product description
General Data 1

Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed

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