GNX T7 RNA Polymerase (250U/µL)
Advantages at a glance
- High activity and specificity for T7 promoter sequences
- Suitable for short and long RNA transcripts
- Compatible with PCR products and linearized plasmids (blunt end or 5'-overhang)
- Liquid formulation for immediate use
€487.19
Product number:
M3507.0010
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Delivery time: 3-8 days
Shipment: on wet ice. Stored at -20°C. For laboratory usage only!
Product information "GNX T7 RNA Polymerase (250U/µL)"
High-Purity Enzyme for In Vitro Transcription
GNX T7 RNA Polymerase is a recombinant enzyme derived from bacteriophage T7 and expressed in Escherichia coli. It catalyzes the 5'→3' synthesis of RNA using double-stranded DNA containing a T7 promoter sequence (5'-TAATACGACTCACTATAGG-3') as a template and NTPs (ATP, CTP, GTP, UTP) as substrates for in vitro transcription.
The polymerase is compatible with linear double-stranded DNA templates with either blunt ends or 5'-protruding ends. This makes it suitable for use with both linearized plasmid DNA and PCR products containing a T7 promoter.
Supplied in a convenient liquid format, this enzyme is ideal for a variety of molecular biology applications such as RNA synthesis, RNA interference (RNAi), hybridization, in vitro translation, and the preparation of RNA probes.
The polymerase is compatible with linear double-stranded DNA templates with either blunt ends or 5'-protruding ends. This makes it suitable for use with both linearized plasmid DNA and PCR products containing a T7 promoter.
Supplied in a convenient liquid format, this enzyme is ideal for a variety of molecular biology applications such as RNA synthesis, RNA interference (RNAi), hybridization, in vitro translation, and the preparation of RNA probes.
Key Features:
- High activity and specificity for T7 promoter sequences
- Suitable for short and long RNA transcripts
- Compatible with PCR products and linearized plasmids (blunt end or 5'-overhang)
- Liquid formulation for immediate use
Synthesis principle:
Specifications:
Source: Recombinantly produced in Escherichia coli
Concentration: mind. 250U/µL
Storage: The enzyme is stable upon storage at - 20°C for up to 1 year
Applikation / Application:
In vitro RNA TranscriptionEinheiten / Units:
The amount of enzyme required to incorporate 1 nmol of [3H] GMP into the acid-insoluble precipitate within 1 hour at 37°C and pH 8.0 is defined as 1 unit.Quelle / Source:
recombinantKlassifizierungen / Classification
eclass No.:
32-16-05-90
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.