GNX T7 RNA Polymerase - low dsRNA (250U/µL)
Advantages at a glance
- Engineered for low dsRNA contamination
- Suitable for both short and long RNA transcripts
- Compatible with blunt-end and 5' overhang DNA
- High RNA yields with excellent specificity
- Ideal for sensitive applications such as in vitro translation, RNAi, and vaccine research
Product number:
M3517.0010
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.
Shipment: on wet ice. Stored at -20°C. For laboratory usage only!
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.
Shipment: on wet ice. Stored at -20°C. For laboratory usage only!
Product information "GNX T7 RNA Polymerase - low dsRNA (250U/µL)"
Optimized Enzyme for In Vitro Transcription
GNX T7 RNA polymerase - low dsRNA is a recombinant enzyme expressed in Escherichia coli and is a genetically modified version of the wild-type T7. The mutation significantly reduces the formation of double-stranded RNA (dsRNA) during transcription, making it ideal for applications requiring high RNA purity.
The polymerase synthesizes RNA in the 5'→3' direction using double-stranded DNA containing a T7 promoter sequence as a template. NTPs (ATP, CTP, GTP, UTP) serve as substrates. Both linear DNA with blunt ends or 5' overhangs can be used as templates – making this enzyme compatible with linearized plasmids and PCR products for efficient in vitro transcription.
Key Benefits:
- Engineered for low dsRNA contamination
- Suitable for both short and long RNA transcripts
- Compatible with blunt-end and 5' overhang DNA
- High RNA yields with excellent specificity Ideal for sensitive applications such as in vitro translation, RNAi, and vaccine research
This polymerase is the perfect choice for precise, efficient, and clean RNA synthesis in molecular biology workflows.
Synthesis Principle:
Specifications:
Source: Recombinantly produced in Escherichia coli
Concentration: mind. 250U/µL
Storage: The enzyme is stable upon storage at - 20°C for up to 1 year
Applikation / Application:
In vitro RNA Transcription
Einheiten / Units:
The amount of enzyme required to incorporate 1 nmol of [3H] GMP into the acid-insoluble precipitate within 1 hour at 37°C and pH 8.0 is defined as 1 unit.
Quelle / Source:
recombinant
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-05-90
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.
Documents:
Manuals
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed
Source: NCBI PubMed