Pwo proofreading DNA polymerase
Advantages at a glance
- 10x Higher Accuracy: Amplifies with 10x greater accuracy than Taq polymerase.
- High-Fidelity: Ensures precise DNA amplification.
- Proofreading Activity: 3'-5' exonuclease for error correction.
- Thermostable: Stable and reliable at high temperatures.
- Blunt-End PCR Products: Ideal for cloning applications.
- Perfect for Cloning & Expression: Produces high-quality PCR products.
Only a few suppliers left worldwide- and we're one of them!Pwo Polymerase: the classic, still precise.
| Quantity | Unit price |
|---|---|
| To 2 |
€35.00
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| To 9 |
€24.50
€35.00
(30% saved)
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| From 10 |
€21.00
€35.00
(40% saved)
|
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Product information "Pwo proofreading DNA polymerase"
The High-Fidelity Pwo proofreading DNA polymerase from Genaxxon bioscience is a thermostable, highly processive enzyme possessing 5'-3' DNA polymerase with additional 3'-5' proofreading exonuclease activity, which enables the correction of nucleotide incorporation errors. It has no 5'→3' exonuclease activity. The High-Fidelity Pwo DNA polymerase is a recombinant form of the hyperthermophilic archaebacteria Pyrococcus woesei.
Pwo proofreading DNA polymerase shows an increased thermostability and a 10-times higher accuracy compared to Taq DNA polymerase. A mixture of Taq DNA Polymerase and Pwo DNA polymerase provides more robust synthesis of longer amplification products (Barnes, 1994. Proc. Natl. Acad. Sci. USA 91:2216-2220).
Test sample available at a special price! The test sample price will be refunded on the first official order of the product.
Features:
- 10-times higher accuracy compared to Taq DNA polymerase
- High-Fidelity polymerase
- Proofreading function (3' - 5' exonuclease activity)
- High thermo stability
- Generates blunt-end PCR products
- Generates PCR products for cloning and expression
More High-Fidelity Proofreading Polymerases from Genaxxon bioscience:
- M3003 ReproFast Proofreading Polymerase
- M3004 Pfu Proofreading Polymerase
- M3012 ReproHot (KOD) Proofreading Polymerase
- AQ97 High Fidelity proofreading Polymerase
With our high quality dNTPs as Set (M3015) or Mix (M3016) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional products for your PCR.
Activity: 2.5 units/µL
Proofreading polymerase (3' - 5' exonuklease activity)
Applikation / Application:
Proof-reading DNA polymerase for high fidelity PCR.Einheiten / Units:
One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C), 50 mMQuelle / Source:
recombinantKlassifizierungen / Classification
Documents:
Safety Data SheetProtocols
Certificate
Manuals
Category List
Cycle times, especially extension times, should be extended, compared to Taq DNA polymerase.
Note:
Recommended elongation time is 1 minute per 250bp of target.
Source: NCBI PubMed
Systematic evaluation of error rates and causes in short samples in next-generation sequencing.
Franziska Pfeiffer, Carsten Gröber, Michael Blank, Kristian Händler, Marc Beyer, Joachim L Schultze, Günter Mayer
Sci Rep. 2018 Jul 19;8(1):10950.
doi: 10.1038/s41598-018-29325-6.
PMCID: PMC6053417.
HHV-8-unrelated primary effusion-like lymphoma associated with clonal loss of inherited chromosomally-integrated human herpesvirus-6A from the telomere of chromosome 19q
Enjie Zhang, Victoria E. Cotton, Alberto Hidalgo-Bravo, Yan Huang, Adam J. Bell, Ruth F. Jarrett, Gavin S. Wilkie, Andrew J. Davison, Ellie P. Nacheva, Reiner Siebert, Aneela Majid, Inga Kelpanides, Sandrine Jayne, Martin J. Dyer, Nicola J. Royle
Sci Rep. 2016; 6: 22730.
doi: 10.1038/srep22730
PMCID: PMC4779988
Human telomeres that carry an integrated copy of human herpesvirus 6 are often short and unstable, facilitating release of the viral genome from the chromosome
Yan Huang, Alberto Hidalgo-Bravo, Enjie Zhang, Victoria E. Cotton, Aaron Mendez-Bermudez, Gunjan Wig, Zahara Medina-Calzada, Rita Neumann, Alec J. Jeffreys, Bruce Winney, James F. Wilson, Duncan A. Clark, Martin J. Dyer, Nicola J. Royle
Nucleic Acids Res. 2014 Jan 1; 42(1): 315–327.
doi: 10.1093/nar/gkt840
PMCID: PMC3874159
Structural and Kinetic Analysis of Bacillus subtilis N-Acetylglucosaminidase Reveals a Unique Asp-His Dyad Mechanism
Silke Litzinger, Stefanie Fischer, Patrick Polzer, Kay Diederichs, Wolfram Welte, Christoph Mayer
J Biol Chem. 2010 Nov 12; 285(46): 35675–35684.
doi: 10.1074/jbc.M110.131037
PMCID: PMC2975192
Muropeptide Rescue in Bacillus subtilis Involves Sequential Hydrolysis by β-N-Acetylglucosaminidase and N-Acetylmuramyl-l-Alanine Amidase
Silke Litzinger, Amanda Duckworth, Katja Nitzsche, Christian Risinger, Valentin Wittmann, Christoph Mayer
J Bacteriol. 2010 Jun; 192(12): 3132–3143.
doi: 10.1128/JB.01256-09
PMCID: PMC2901692
Pfu DNA Polymerase – Precision, Robustness, and Cost-Efficiency in One Enzyme
Proofreading Polymerases and Master Mixes - Achieve Results Quickly and Accurately with GENAXXON
Optimization of Annealing Temperature and other PCR Parameters
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