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Pwo proofreading DNA polymerase

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  • High-quality products
  • Customized solutions
  • Personal contact
  • Fast service
  • Competent technical support +49(0)731-3608-123


Product of the month in JUNE!
and Pfu proofreading Polymerase >

Übersicht Genaxxon
proofreading Polymerasen
und Anwendungen >

 

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Product number: M3002.0100
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Shipment on wet ice. Store at -20°C. For laboratory usage only!
Product information "Pwo proofreading DNA polymerase"

The High-Fidelity Pwo proofreading DNA polymerase from Genaxxon bioscience is a thermostable, highly processive enzyme possessing 5'-3' DNA polymerase with additional 3'-5' proofreading exonuclease activity, which enables the correction of nucleotide incorporation errors. It has no 5'→3' exonuclease activity. The High-Fidelity Pwo DNA polymerase is a recombinant form of the hyperthermophilic archaebacteria Pyrococcus woesei.

Pwo proofreading DNA polymerase shows an increased thermostability and a 10-times higher accuracy compared to Taq DNA polymerase >. A mixture of Taq DNA Polymerase and Pwo DNA polymerase provides more robust synthesis of longer amplification products (Barnes, 1994. Proc. Natl. Acad. Sci. USA 91:2216-2220).

Test sample available at a special price! The test sample price will be refunded on the first official order of the product.

Features:

  • 10-times higher accuracy compared to Taq DNA polymerase
  • High-Fidelity polymerase
  • Proofreading function (3' - 5' exonuclease activity)
  • High thermo stability
  • Generates blunt-end PCR products
  • Generates PCR products for cloning and expression

More High-Fidelity Proofreading Polymerases from Genaxxon bioscience:

- M3002 Pwo Proofreading Polymerase >
- M3003 ReproFast Proofreading Polymerase >
- M3004 Pfu Proofreading Polymerase >
- M3012 ReproHot (KOD) Proofreading Polymerase >
- M3030 ExactRun (Phusion like) Proofreading Polymerase >

With our high quality dNTPs as Set (M3015.4100 and M3015.0250) > or Mix (M3016.1010) > or our DNA Ladders > and our favourable standard agarose (M3044) > we can offer additional products for your PCR.

FAQs
The extension rate of the Pwo or Pfu is lower than that of a standard Taq (up to 4 times). Therefore, it is a common phenomenon that the bands are weaker when using Pwo or Pfu compared to a standard Taq. To obtain a higher yield of amplified DNA with Pwo or Pfu, you should increase the extension time up to twice the time you need with a standard Taq.
Specificaitons:
Activity: 2.5 units/µL
Proofreading polymerase (3' - 5' exonuklease activity)

 

 


Applikation / Application:
Proof-reading DNA polymerase for high fidelity PCR.

Einheiten / Units:
One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C), 50 mM

Quelle / Source:
recombinant


Sicherheits Hinweise / Safety


Klassifizierungen / Classification

eclass-Nr: 32-16-05-02
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.


Documents:

Safety Data Sheet
Protocols
Certificate
Manuals
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed

Quelle/Source: NCBI PubMed >


Systematic evaluation of error rates and causes in short samples in next-generation sequencing

Franziska Pfeiffer, Carsten Gröber, Michael Blank, Kristian Händler, Marc Beyer, Joachim L. Schultze, Günter Mayer

Sci Rep. 2018; 8: 10950. Published online 2018 Jul 19. doi: 10.1038/s41598-018-29325-6

PMCID: PMC6053417

 

HHV-8-unrelated primary effusion-like lymphoma associated with clonal loss of inherited chromosomally-integrated human herpesvirus-6A from the telomere of chromosome 19q

Enjie Zhang, Victoria E. Cotton, Alberto Hidalgo-Bravo, Yan Huang, Adam J. Bell, Ruth F. Jarrett, Gavin S. Wilkie, Andrew J. Davison, Ellie P. Nacheva, Reiner Siebert, Aneela Majid, Inga Kelpanides, Sandrine Jayne, Martin J. Dyer, Nicola J. Royle

Sci Rep. 2016; 6: 22730.  Published online 2016 Mar 7. doi: 10.1038/srep22730

PMCID: PMC4779988

 

Human telomeres that carry an integrated copy of human herpesvirus 6 are often short and unstable, facilitating release of the viral genome from the chromosome

Yan Huang, Alberto Hidalgo-Bravo, Enjie Zhang, Victoria E. Cotton, Aaron Mendez-Bermudez, Gunjan Wig, Zahara Medina-Calzada, Rita Neumann, Alec J. Jeffreys, Bruce Winney, James F. Wilson, Duncan A. Clark, Martin J. Dyer, Nicola J. Royle

Nucleic Acids Res. 2014 Jan 1; 42(1): 315–327.  Published online 2013 Sep 19. doi: 10.1093/nar/gkt840

PMCID: PMC3874159

 

Structural and Kinetic Analysis of Bacillus subtilis N-Acetylglucosaminidase Reveals a Unique Asp-His Dyad Mechanism

Silke Litzinger, Stefanie Fischer, Patrick Polzer, Kay Diederichs, Wolfram Welte, Christoph Mayer

J Biol Chem. 2010 Nov 12; 285(46): 35675–35684.  Published online 2010 Sep 7. doi: 10.1074/jbc.M110.131037

PMCID: PMC2975192

 

Muropeptide Rescue in Bacillus subtilis Involves Sequential Hydrolysis by β-N-Acetylglucosaminidase and N-Acetylmuramyl-l-Alanine Amidase

Silke Litzinger, Amanda Duckworth, Katja Nitzsche, Christian Risinger, Valentin Wittmann, Christoph Mayer

J Bacteriol. 2010 Jun; 192(12): 3132–3143.  Published online 2010 Apr 16. doi: 10.1128/JB.01256-09

PMCID: PMC2901692


Cycle times, especially extension times, should be extended, compared to Taq DNA polymerase.
Note:
Recommended elongation time is 1 minute per 250bp of target.

Proofreading Polymerases and Master Mixes - Achieve Results Quickly and Accurately with GENAXXON

High Fidelity Proofreading DNA Polymerases offer high amplification rates combined with exceptional accuracy

Optimization of Annealing Temperature and other PCR Parameters

There are many different suppliers and systems for polymerase chain reaction (PCR)