TEMPase Hot Start DNA Polymerase
Advantages at a glance
- High-quality products
- Customized solutions
- Personal contact
- Fast service
- Competent technical support +49(0)731-3608-123
€90.00
- Free shipping
- Shipping within 24h
- 30 days money back guarantee
- Purchase comfortable on account
Delivery time: 3-8 days
Product information "TEMPase Hot Start DNA Polymerase"
About TEMPase Hot Start DNA Polymerase
TEMPase Hot Start DNA Polymerase has been designed to avoid spurious amplification and to diminish the formation of non specific priming events during your set up. TEMPase Hot Start DNA Polymerase allows for detection of low copy number targets and for multiplex PCR in a single reaction tube. TEMPase Hot Start DNA Polymerase is available together with different buffers. This one (A2251xx) is supplied with 10X ammonium sulfate buffer plus 10X combination buffer plus separate 25mM MgCl2.
TEMPase Hot Start DNA Polymerase is also available together with 10X ammonium sulfate buffer and separate 25mM MgCl2 (A2211xx).
Features
- Reaction set up at room temperature
- Increased sensitivity
- Increased specificity
- Increased product yield
Description
TEMPase Hot Start DNA Polymerase is a modified form of Ampliqon Taq DNA Polymerase, which is activated by heat treatment.
A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during set up and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended.
Once the reaction reaches optimal activating temperature, the chemical moiety is cleaved during a heat activation step, releasing the active TEMPase Hot Start DNA Polymerase into the reaction.
Please check also our SuperHot Hot Start Mastermix > with or without tracking dye.
With our high quality dNTPs as Set (M3015.4100 and M3015.0250) > or Mix (M3016.1010) > or our DNA Ladders > and our favourable standard agarose (M3044) > we can offer additional products for your PCR.
Documents:
ManualsGeneral Data 4
Category List
