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dNTP-Mix (Na-salt) - 2 mM - ready to use

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Genaxxon's dNTP Mix 2mM Ready to use

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€25.00

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Product number: M3017.1002
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Immediately available, Delivery time: 1-2 days

Product information "dNTP-Mix (Na-salt) - 2 mM - ready to use"

Highly pure, HPLC purified dNTPs (>99%) delivered as a 2 mM solution of dATP, dCTP, dGTP and dTTP for use in qPCR, standard PCR, RT-PCR and Klenow reactions. Use 5 µL of Mix for PCR in 50µL reaction volume.
The Genaxxon dNTP mix is optimized for its use in DNA polymerisation and related methods. Our dNTPs contain no measurable bacterial or human DNA. For storage for a prolonged periode of time we recommend to prepare small aliquots, especially in case of rare use.

  • Mixture of dNTP sodium salt solutions (dATP, dCTP, dGTP und dTTP)
  • Concentration: 2 mM of ach nucleotide

Please have also a look on our broad range of nucleotides especially the dNTP mix with 10 mM dNTP mix, our dNTP set , or modified nucleotides, e.g. Biotin-11-dUTP. 

You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001), or our proof-reading polymerases Pfu (M3004), Pwo (M3002) and ReproFast (M3003), as well as the ready-to-use RedMastermix (M3029), already including dNTPs.

FAQs
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dNTPs (deoxynucleoside triphosphates) are the building blocks of DNA. In both PCR and qPCR, the quality, stability, and quantity of dNTPs are crucial. Ensuring their purity is particularly vital when you're dealing with small starting amounts of DNA template.
Genaxxon offers dNTPs in two different forms: either as a set or a premixed solution. The mix conveniently provides all four types—dATP, dCTP, dGTP, and dTTP—in a single tube, each at a specific concentration. These mixes are designed for immediate use, meticulously fine-tuned for PCR and qPCR tasks. On the other hand, the dNTP set offers individual tubes for each dNTP, providing flexibility and control over their usage.
Typically, a standard concentration for each dNTP in a PCR setup is 0.2mM (concentrations from 0.2mM to 0.4mM are usually ideal for your PCR). Lower concentrations, between 0.05mM to 0.1mM, may enhance PCR specificity but could reduce product yield. Conversely, higher concentrations may boost yield but at the expense of specificity. Longer PCR fragments might necessitate higher dNTP concentrations. However, adjusting dNTP concentration requires corresponding adjustments in other PCR components like buffer and Mg2+.
Typically, increasing the amount of dNTPs in your PCR can enhance DNA yield but might compromise specificity. Please note that it’s also necessary adjusting other PCR components such as buffer and Mg2+ if you increase the dNTP concentration.
The PCR assay can be significantly impacted by inhibitors present in dNTP solutions that are not ultra-pure, often stemming from inadequate manufacturing processes. Ideally, dNTPs should be free of impurities such as ribonucleoside triphosphates, additional dNTPs, modified nucleotides, deoxynucleoside di- and monophosphates, heavy transition metals, or other inhibitors.
Low-quality dNTPs may contain modified nucleotides. When using a standard Taq polymerase, point mutations may occur because the enzyme cannot distinguish between different dNTPs. Using a proofreading polymerase can only partially address this issue, as the presence of modified nucleotides can also completely block DNA synthesis.
dNTPs remain stable when stored at -20°C or -70°C in a consistent-temperature freezer for at least 24 months. If stored at room temperature (+15°C to +30°C), they can be kept for up to one week. To maintain their stability, it's best to avoid repeated thawing and freezing cycles. For prolonged storage, it's advisable to use aliquots.
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.


Documents:

Safety Data Sheet
Certificate
Product description
General Data 1
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed
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High-quality products
Customized solutions
Personal contact
Fast service
Competent technical support +49(0)731-3608-123
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