NGSClone PCR MasterMix (2X)
Advantages at a glance
- High fidelity with fusion enzyme characteristics: The NGSClone DNA polymerase performs with high fidelity and has a high amplification ability.
- Hot-Start function: Blocked with an antibody, the enzyme has a high specificity and does not operate at low temperatures.
- Fast amplification – achieves target amplification in just 10–15 seconds per kb
- Wide applicability: Also effective for GC-rich templates.
- Ready-to-use: The master mix includes all components for your reliable PCR. You only need to add primers and DNA.
€299.75
Product number:
M3139.0400
Our benefits
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Delivery time: 3-8 days
Shipment: on wet ice. Stored at -20°C. For laboratory usage only!
Product information "NGSClone PCR MasterMix (2X)"
The Genaxxon NGSClone PCR Mastermix (2x) combines high-fidelity DNA polymerase with antibody-mediated Hot-Start technology for precise, fast, and reliable PCR results. Perfect for challenging templates such as GC-rich DNA, cDNA, or complex plant and animal samples. This ready-to-use master mix contains all essential components – just add primers and template – making it ideal for high-fidelity PCR and NGS library preparation.
Genaxxon’s NGSClone PCR Mastermix (2x) – for fast, precise, and reproducible PCR results with maximum convenience.
NGSClone PCR Mastermix (2x) – High-Fidelity & Hot-Start for Accurate PCR
Maximum accuracy for demanding PCR applications
The Genaxxon NGSClone PCR Mastermix (2x) delivers outstanding performance by combining a novel high-fidelity DNA polymerase with antibody-mediated Hot-Start technology in a convenient ready-to-use PCR master mix. With up to 120× higher fidelity compared to standard Taq polymerases, it ensures highly accurate amplification even for GC-rich templates. Ideal for PCR in NGS workflows, cDNA amplification, and plant or animal DNA analysis.
Key Features of the NGSClone PCR Mastermix (2x)
- Exceptional accuracy – High-fidelity DNA polymerase with strong 3’-5’ exonuclease activity (proofreading) for error-free amplification
- Fast & efficient PCR – Engineered enzyme structure with enhanced domain for high processivity and faster extension rates
- Fast amplification – achieves target amplification in just 10–15 seconds per kb
- Hot-Start precision – Antibody blocking keeps the enzyme inactive at room temperature, preventing non-specific amplification and improving specificity
- Broad applicability – Suitable for GC-rich DNA, cDNA, plant and animal templates, and other difficult targets
- Easy workflow – Contains polymerase, dNTPs, and optimized buffer; just add primers and DNA
Benefits in Your Lab
- Saves time and reduces pipetting steps
- Minimizes cross-contamination
- Delivers high reproducibility
- Remains stable after repeated freeze-thaw cycles
Applications
- High-fidelity PCR for error-free cloning
- PCR of GC-rich templates
- cDNA amplification
- NGS library preparation
- Standard PCR requiring high accuracy
FAQs
Possible issues
- Primer or DNA template concentration is too high. Solution: Reduce the amount of primers and template appropriately.
- Annealing temperature is too low or too many PCR cycles. Solution: Increase annealing temperature or decrease cycle number by performing gradient optimization.
- PCR primer mismatch. Solution: Redesign PCR primers.
Possible issues
- Problems with PCR primers. Solution: Set up a positive control reaction to check primer functionality.
- Annealing temperature is not optimal. Solution: Adjust the annealing temperature and perform gradient optimization experiments.
- Low primer concentration or insufficient PCR cycle number. Solution: Increase the amount of primers or the number of amplification cycles.
- Insufficient extension time. Solution: Extend the extension time to about 15 s/kb.
- Presence of inhibitors in the template. Solution: Use a high-purity template or dilute the template before amplification.
Specifications:
2-time ready-to-use master mix for for high fidelity PCR with excellent processivity. dNTPs, buffer and DNA polymerase already included.
Convenient Aliquot size: 1mL
Klassifizierungen / Classification
eclass No.:
32-16-05-02
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.
Documents:
Product descriptionWhy is next generation sequencing called so?
April 20, 2020
Science
Sanger sequencing technology was the first generation of sequencing and helped provide basic knowledge
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