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RedMasterMix Fluoro (2x) with gel staining dye

Advantages at a glance

  • No post-staining processing of nucleic acid 
  • Direct loading onto agarose gel for electrophoresis without adding a loading buffer 
  • High degree of sensitivity (comparable to ethidium bromide) 
  • No destaining requirement 
  • Use the blue light or UV to detect the signal

€69.00*

Volume
Product number: M3236.0100
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.

Shipment: on wet ice. Store at -20°C after delivery. For laboratory usage only!
Product information "RedMasterMix Fluoro (2x) with gel staining dye"

ready-to-use PCR Mastermix with red Loading Dye for visual control of the pipetting steps and additional fluorescent dye for fast and easy detection of the DNA bands. After PCR, the PCR mix can be pipetted directly into the gel pockets without adding loading buffer. This makes our RedMasterMix Fluoro (2x) even more time- and cost-saving than our proven Red Mastermix. The RedMasterMix Fluoro (2x) with fluorescent gel staining dye and red loading dye also features high specificity for best results. The RedMasterMix Fluoro (2x) is a ready-to-use mixture of:

- Taq DNA Polymerase
- PCR reaction buffer
- dNTPs
- MgCl2
- red loading dye
- fluorescence gel staining dye for DNA band detection

in an optimal concentration for efficient amplification of DNA templates by PCR. Only the primers and the template DNA have to be added. At the same time, the PCR Matermix contains an additive and a red dye, which allows subsequent electrophoresis without the addition of loading buffer. After electrophoresis, detection is performed directly under blue light without further staining. This saves additional time and costs. The RedMasterMix Fluoro (2x) with fluorescent dye was developed for use in routine PCR up to 4 kb amplicon length. The special composition of the buffer guarantees reproducible results even after repeated thawing and freezing cycles. Our Red MasterMix (2x) Fluoro is shipped in convenient aliquots of 1.25mL.

For more information on our SimplyEnlight PCR products, read our blog now. Genaxxon's SimplyEnlight PCR - Unlock the Power of Your PCR!

FAQs
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Yes, the integrated fluorescent dye DNA loading buffer I Fluoro (6x) is a non-toxic nucleic acid dye. It can be easily disposed of with normal waste. Separate handling of the waste as with EtBr is therefore not necessary. If necessary, obtain information from your local waste disposal company.
If you want to benefit from all the advantages of the SimplyEnlight PCR products, you will also need to use a DNA ladder with fluorescent dye to avoid further staining of the gel. To make it as convenient as possible for you, you can use our GenLadder 100bp Plus with staining dye for this purpose. However, you can take our DNA Ladder 100bp (or any other) and add our DNA Loading Buffer I Fluoro (6X) in a 5:1 ratio before loading the gel.
Yes, you can use your own cycling conditions. Please note that the cycling conditions in our protocol are only guidelines for PCR amplification. Optimal reaction conditions such as incubation times, temperatures, and amount of template DNA may vary and must be determined individually. Besides this, optimization of the annealing temperature is important to guarantee an optimal amplification. We recommend performing a temperature gradient and using primers with a Tm >60°C (Tm = melting temperature of the primer, which is the temperature at which 50% of the primer bind to the complementary sequence of the target DNA.)
If DNA concentration is less than 4pg, it may cause a migratory shift when performing gel electrophoresis. To remedy this, we recommend removing the fluorescent dye prior to post-staining with our DNA Loading buffer I Fluoro (6x) again for restoring the DNA molecular weight in the original position. To remove the fluorescent dye, immerse the PCR product containing the fluorescent dye into 100mM NaCl and add 2.5 volumes of absolute or 95% ethanol. Incubate on ice for 20 min and centrifuge the mixture at 4°C for at least 10 minutes. Then remove the suspension of ethanol and wash the pellet with 1mL of 70% ethanol. Dry the residual ethanol and resuspend the dsDNA in the TE buffer.

Specifications:
2-time ready-to-use master mix for PCR including a red dye for better visualization of pipetting and as gel loading dye and a fluorescence dye for detection of DNA bands in an electrophoresis gel. dNTPs, buffer and DNA polymerase already included.
Buffer contains orange G & xylene cyanol FF as tracking dyes.
Buffer contains a fluorescent gel staining dye for visualization of DNA bands.

Convenient Aliquot size: 1.25mL.

This master mix is stable for up to 3 months if stored at +15°C to +30°C.
This master mix is stable for up to 6 months if stored at +2°C to +8°C.
This master mix is stable for up to 24 months if stored at -20°C.


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Klassifizierungen / Classification

eclass-Nr: 32-16-05-02
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.


Documents:

Manuals
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed