RedMasterMix Fluoro (2x) with gel staining dye
Advantages at a glance
- No post-staining processing of nucleic acid
- Direct loading onto agarose gel for electrophoresis without adding a loading buffer
- High degree of sensitivity (comparable to ethidium bromide)
- No destaining requirement
- Use the blue light or UV to detect the signal
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.
Shipment: on wet ice. Store at -20°C after delivery. For laboratory usage only!
ready-to-use PCR Mastermix with red Loading Dye for visual control of the pipetting steps and additional fluorescent dye for fast and easy detection of the DNA bands. After PCR, the PCR mix can be pipetted directly into the gel pockets without adding loading buffer. This makes our RedMasterMix Fluoro (2x) even more time- and cost-saving than our proven Red Mastermix. The RedMasterMix Fluoro (2x) with fluorescent gel staining dye and red loading dye also features high specificity for best results. The RedMasterMix Fluoro (2x) is a ready-to-use mixture of:
- Taq DNA Polymerase
- PCR reaction buffer
- dNTPs
- MgCl2
- red loading dye
- fluorescence gel staining dye for DNA band detection
in an optimal concentration for efficient amplification of DNA templates by PCR. Only the primers and the template DNA have to be added. At the same time, the PCR Matermix contains an additive and a red dye, which allows subsequent electrophoresis without the addition of loading buffer. After electrophoresis, detection is performed directly under blue light without further staining. This saves additional time and costs. The RedMasterMix Fluoro (2x) with fluorescent dye was developed for use in routine PCR up to 4 kb amplicon length. The special composition of the buffer guarantees reproducible results even after repeated thawing and freezing cycles. Our Red MasterMix (2x) Fluoro is shipped in convenient aliquots of 1.25mL.
Specifications:
2-time ready-to-use master mix for PCR including a red dye for better visualization of pipetting and as gel loading dye and a fluorescence dye for detection of DNA bands in an electrophoresis gel. dNTPs, buffer and DNA polymerase already included.
Buffer contains orange G & xylene cyanol FF as tracking dyes.
Buffer contains a fluorescent gel staining dye for visualization of DNA bands.
Convenient Aliquot size: 1.25mL.
This master mix is stable for up to 3 months if stored at +15°C to +30°C.
This master mix is stable for up to 6 months if stored at +2°C to +8°C.
This master mix is stable for up to 24 months if stored at -20°C.
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-05-02
Documents:
ManualsSource: NCBI PubMed