SuperHot Multiplex Mastermix (2X)
Advantages at a glance
- 2-time ready-to-use multiplex master mix
- With a chemically modified Taq DNA polymerase
- Without green fluorescent dye
- Without ROX™
€895.00
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Delivery time: 3-8 days
Product information "SuperHot Multiplex Mastermix (2X)"
Multiplex PCR is a method that enables amplification of two or more amplicons simultaneously in a single reaction tube/reaction. It is widely used in genotyping and different areas of DNA testing in research, forensic and diagnostic laboratories.
Our SuperHot Multiplex Mastermix (2X) is an optimized ready-to-use mixture for probe-based assays such as TaqMan®, Beacons and MGBs. It contains a modified fast HotStart Taq DNA Polymerase, dNTPs and MgCl₂ combined in an optimized buffer system for realtime PCR / qPCR applications except primers, probe and template DNA / cDNA.
The SuperHotStart Taq polymerase contained in our SuperHot Multiplex Mastermix is our tried-and-tested SuperHot Taq DNA polymerase M3307 >, whose polymerase activity has been inactivated by a chemical modification at the active site. To activate the polymerase, it requires a 10–15-minute activation step at 94°C. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity, increased sensitivity and greater yields when compared to standard DNA polymerases, making this enzyme especially well-suited for multiplex PCR.
- Amplification of multiple targets in a single tube
- All-in-one master mix for convenient multiplexing
- High specificity, sensitivity and product yield
- Easy reaction setup at room temperature
The SuperHot Multiplex Master mix 2X is shipped in aliquots of 1.25mL.
Our Standard Agarose LE > and especially our high resolution Agarose Tiny > are ideally suited for the subsequent electrophoresis analysis.
Other realtime master mixes for your realtime PCR experiments can be found here >.
Examples of Multiplex applications:
F. Javier Pérez-Pérez and Nancy D. Hanson, Detection of Plasmid-Mediated AmpC β-Lactamase Genes in Clinical Isolates by Using Multiplex PCR, J. Clin. Microbiol. June 2002 vol. 40 no. 6 2153-2162. doi: 10.1128/JCM.40.6.2153-2162.2002.
Specifications:
2-time ready-to-use multiplex master mix
with a chemically modified Taq DNA polymerase
without green fluorescent dye
without ROX™