Random Hexamer Primer N6
Advantages at a glance
- Purification: desalted (HPLC purified and MS checked)
- Amount: 76.1nmol / 136.4µg / 5 OD
- Primer sequence: randomized Sequence [d(N)6]
| Quantity | Unit price |
|---|---|
| To 2 |
€28.90
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| To 5 |
€25.99
€28.90
(10.07% saved)
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| To 9 |
€24.55
€28.90
(15.05% saved)
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| From 10 |
€21.66
€28.90
(25.05% saved)
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Immediately available, Delivery time: 1-2 days
Product information "Random Hexamer Primer N6"
Random Hexamers are short oligodeoxyribonucleotides of random sequence [d(N)6]. Primers are quality controlled (MS), purified (reversed phase HPLC), lyophilised and aliquoted and came with a guaranteed amount of at least 5OD (about 76.1nmol (136.4µg)).
Hexanucleotide primers are a mixture of random 5'-hydroxyl hexanucleotides or hexamers, and can be used to quickly and efficiently prepare radioactive or non-radioactive probes using a DNA polymerase and a suitable DNA template or for cDNA synthesis from mRNA. The heterogeneous nature of the random primers ensures that all possible sequences will be represented in the probe mixture. As a result, these primers are well suited for generating as long as possible (complete) transcripts of the RNA, transcribing the 5 'ends, in particular for long RNA templates.
As an alternative you can also use Oligo(dT)-Primer > for reverse transcription. Oligo(dT) primer bind to the polyA tail of RNA and therefore transcription will start always at the 3'-end of the RNA.
The guaranteed delivery quantity of 5OD, corresponding to approx. 76.1nmol, or 136.4μg, can be dissolved in 760μL DEPC water to give a concentration of 100pmol/μL (100μM), or approx. 180μg/μL. If using between 50ng and 250ng of the hexamers per 20μL reverse transcription reaction, 760μL (100μM) will be sufficient for 460 to 2280 reverse transcriptase reactions.
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