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DNA Loading buffer I Fluoro (6x)

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Advantages at a glance

  • Highly sensitive
  • Simple to use
  • Timesaving
  • Perfectly compatible with a bluelight detector
  • Easy disposal

€25.00

Volume
Product number: M3323.0001
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Immediately available, Delivery time: 1-2 days

Shipment: not cooled. Stored at -20°C. For laboratory usage only!

Product information "DNA Loading buffer I Fluoro (6x)"

Loading buffer I Fluoro is a fluorescent reagent that produces instant visualization of DNA bands upon blue light or UV illumination of agarose gels. Supplied in 6X DNA Loading Buffer, Loading buffer I Fluoro is used to prepare DNA markers or samples for loading on agarose or polyacrylamide gels. 

Loading buffer I Fluoro is the sensitive staining reagent available for detecting the double-stranded DNA (dsDNA). It contains three tracking dyes (bromophenol blue, xylene cyanol FF, and orange G) for visually tracking the DNA migration during the electrophoresis plus an additional fluorescence dye for detection of DNA bands without an additional staining of the gel. 

Please note: DNA Loading Buffer I Fluoro does show a small excitation peak in the UV range, but fluorescence is significantly stronger under blue light excitation (ca. 490nm). We therefore recommend using blue light to achieve the best possible results.

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FAQs
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Yes, the integrated fluorescent dye DNA loading buffer I Fluoro (6x) is a non-toxic nucleic acid dye. It can be easily disposed of with normal waste. Separate handling of the waste as with EtBr is therefore not necessary. If necessary, obtain information from your local waste disposal company.
If you want to benefit from all the advantages of the SimplyEnlight PCR products, you will also need to use a DNA ladder with fluorescent dye to avoid further staining of the gel. To make it as convenient as possible for you, you can use our GenLadder 100bp Plus with staining dye for this purpose. However, you can take our DNA Ladder 100bp (or any other) and add our DNA Loading Buffer I Fluoro (6X) in a 5:1 ratio before loading the gel.
Yes, you can use your own cycling conditions. Please note that the cycling conditions in our protocol are only guidelines for PCR amplification. Optimal reaction conditions such as incubation times, temperatures, and amount of template DNA may vary and must be determined individually. Besides this, optimization of the annealing temperature is important to guarantee an optimal amplification. We recommend performing a temperature gradient and using primers with a Tm >60°C (Tm = melting temperature of the primer, which is the temperature at which 50% of the primer bind to the complementary sequence of the target DNA.)
If DNA concentration is less than 4pg, it may cause a migratory shift when performing gel electrophoresis. To remedy this, we recommend removing the fluorescent dye prior to post-staining with our DNA Loading buffer I Fluoro (6x) again for restoring the DNA molecular weight in the original position. To remove the fluorescent dye, immerse the PCR product containing the fluorescent dye into 100mM NaCl and add 2.5 volumes of absolute or 95% ethanol. Incubate on ice for 20 min and centrifuge the mixture at 4°C for at least 10 minutes. Then remove the suspension of ethanol and wash the pellet with 1mL of 70% ethanol. Dry the residual ethanol and resuspend the dsDNA in the TE buffer.

Specifications:
6-time loading buffer for agarose gels
contains: Glycerin, EDTA, Bromophenol blue, Xyclene cyanol, Orange G plus a fluorescence dye

Applikation / Application:

for application of DNA onto Agarose and Polyacrylamide gels

Quelle / Source:

synthetic

Klassifizierungen / Classification

eclass No.: 32-16-05-02
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.


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High-quality products
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