DNA Loading buffer I Fluoro (6x)
Advantages at a glance
-
Highly sensitive
- Simple to use
- Timesaving
- Perfectly compatible with a standard UV transilluminator or bluelight detector
- Easy disposal
Ready to ship today,
Delivery time 1-2 workdays
Shipment: not cooled. Stored at -20°C. For laboratory usage only!
Loading buffer I Fluoro is a fluorescent reagent that produces instant visualization of DNA bands upon blue light or UV illumination of agarose gels. Supplied in 6X DNA Loading Buffer, Loading buffer I Fluoro is used to prepare DNA markers or samples for loading on agarose or polyacrylamide gels.
Loading buffer I Fluoro is the sensitive staining reagent available for detecting the double-stranded DNA (dsDNA). It contains three tracking dyes (bromophenol blue, xylene cyanol FF, and orange G) for visually tracking the DNA migration during the electrophoresis plus an additional fluorescence dye for detection of DNA bands without an additional staining of the gel. It is an ideal alternative to Ethidium Bromide (EtBr).
Approximate fluorescence excitation / emission: 300, 495 / 537 nm, bound to nucleic acid.
Picture 1: Comparison of UV- and blue light excitation Picture 2: Effect of EtBr plus UV on cloning efficiency.
Specifications:
6-time loading buffer for agarose gels
contains: Glycerin, EDTA, Bromophenol blue, Xyclene cyanol, Orange G plus a fluorescence dye
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-05-02
Documents:
Safety Data SheetProduct description
Source: NCBI PubMed