Chymotrypsinogen A - Chymotrypsin precursor

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Weight

5 g

Product number: S5230.0005
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.

Shipment: not cooled. Stored at -20°C. For laboratory usage only!

Product information "Chymotrypsinogen A - Chymotrypsin precursor"

Chymotrypsinogen is the practically inactive "precursor" (proenzyme, or zymogen) of chymotrypsin. In order to be activated, chymotrypsinogen has to be cleaved by trypsin between the amino acids arginine and isoleucine (R15 and I16) , which leads to structural changes and the formation of the substrate binding site (Sears 2010). Chymotrypsin is a trypsin-like serine protease.

Chymotrypsin differs from trypsin in the selectivity of the cleavage site of proteins. While trypsin cleaves proteins between the amino acids Arg and Lys, Chymotrypsin prefers large hydrophobic amino acids as cleavage site.

Unit definition: The amount of activated zymogen that causes a decrease in absorbance at 237 nm of 0.0075 per minute at 25°C resulting from the hydrolysis of ATEE (NF/USP unit).

Specifications:
pot. Activity: min. 1200 U/mg
native activity: min. 7 U/mg (before cleavage by Trypsin)
MW = about 25,000 g/mol
Optimal pH: 7.8-8.0 (Rick 1974)

Applikation / Application:

- digestion of proteins and peptides - sequence analysis by edman - peptide mapping

Einheiten / Units:

One unit is defined as the amount of enzyme that causes a decrease in absorbance at 237nm of 0.0075 per minute at 25°C resulting from the hydrolysis of ATEE (NF/USP unit).

Quelle / Source:

bovine pancreas

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

EC-Nr: 232-905-3
CAS-Nr: 9035-75-0
eclass-Nr: 32-16-04-10

Documents - Protocols - Downloads :

Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

Documents:

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Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed