rHu HER2-ECD / ErbB2/Her2

HER2/neu/ErbB-2 (human epidermal growth factor receptor 2) is a membrane glycoprotein of the ErbB family of tyrosine kinase receptors. This protein family members (ErbB1-4) serve as receptors for epidermal growth factor. ErbB2 is found on the on the surface of epithelial cells and strongly overexpressed on the membrane of cancer cells especially breast cancer cells. ErbB2 has no identified ligand but rather forms heterodimers with one the other ErbB receptors. These complexes are of high affinity to its ligands. By binding of the ligand a conformational change of the cytoplasmatic tyrosine kinase part of the ErbB2 receptor results in phosphorylation of the initial PI3K/Akt signal transduction cascade protein and effects cell proliferation. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa leader sequence, a 631 aa extracellular domain (ED), a 23 aa transmembrane region, and a 580 aa cytoplasmatic domain. The soluble ED can be shed proteolytically from the cell surface, is strongly glycosylated and of a MW of 95-105 kDa.

Sequence data: TQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSL
TEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHC
PALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPL
QPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCAR
GHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVD
LDDKGCPAEQRASPLTS