LyoBalls in 96-well low profile beakable PCR Platte - pre-aliquoted

Advantages at a glance

Stable at ambient temperature
No cooling required
pre-aliquoted with all required reagents
Minimized risk of contamination
For multiplex PCR recommended

€150.14

pack size

1 Plate

Product number: M3069.0196

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Delivery time: 3-8 days

Shipment: not cooled. Store at +15°C to +30°C. For laboratory usage only!

Product information "LyoBalls in 96-well low profile beakable PCR Platte - pre-aliquoted"

Lyophilized (freeze-dried) temperature stable, pre-mixed and pre-dispensed Hotstart master mix with all necessary components for probe realtime PCR (optimized buffer, dNTPs, Hotstart Taq) for difficult to amplify templates, qPCR and Multiplex applications. Only PCR-grade water has to be added to get the ready-to-use PCR master mix.

While using our LyoBalls there is no need for cooling anymore! The lyoophilized master mix is stable at ambient temperature for at least 24 months if unopened! Our Hotstart Polymerase guarantees that the enzyme is not active during setup and below 55°C at all PCR cycles. Thus misprimed primers are not extended. As a result specificity and efficiency are increased by far compared to standard Taq DNA polymerase. Additionally, difficult targets with high GC-content can be amplified. The higher sensitivity improves multiplex PCR results.

The lyophilized master mix is supplied in a breakable 96-well PCR plate. The plate can be divided into individual "8's strips". Thus, you have the variability to run smaller or larger preparations, depending on your needs.

Each bead is equivalent to one 20µL PCR reaction.

The lyophilized master mix in an easy to handle bead format is the perfect choice for a fast reaction setup that reduces the time required for pipetting and the possibility for pipetting errors. Just add PCR-grade water, template and primers to reconstitute the master mix and you are all set!

Lyophilized realtime PCR master mix:

• Hotstart Polymerase

• increased specificity

• increased sensitivity

• for difficult to amplify templates with high GC content

• for Multiplex applications

• for qPCR

• ready-to-use

Our lyophilized qPCR Probe master mix is also available in powder form (LyoMix, M3071 >).

With our high quality dNTPs as Set > or Mix > or our DNA Ladders > and our favourable standard agarose (M3044 >) we can offer additional products for your PCR.

Specifications:
LyoBalls are a ready-to-use freeze-dried master mix that contains all necessary components for realtime PCR.
Aliquot size: 1 bead = 1 PCR reaction.
Standard pack format: predispensed in a breakable PCR plate (0.1mL wells). Plate easily breakable in strips of 8 wells. Wells closed with 8 cap strips.

This master mix is stable for at least 24 months at ambient temperature if the supplied plate is kept unopened!

Applikation / Application:

For multiplex PCR. For Real-time PCR.For PCR of low amount of DNA (low copy number).

Klassifizierungen / Classification

eclass No.: 32-16-05-02

Documents - Protocols - Downloads :

Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

Documents:

Safety Data Sheet
Protocols
Manuals
General Data 3
Category List

- LyoBalls can look wet. This is due to a specially developed active ingredient that protects the balls from absorbing moisture but does not interfere with the PCR reaction.

- LyoBalls dissolve within seconds at room temperature without further mixing.

- Master mixes, whether liquid or lyophilized, from different companies contain different components, which most likely leads to different annealing and melting behaviour of DNA, primers and probes in your PCR! It is most likely not possible to perform the same PCR protocol with the LyoBalls that has already been defined for another master mix.

- If you use LyoBalls for the first time or create a new primer set, perform a PCR with a temperature gradient in the annealing and extension step to determine the optimal temperature for your assay or each primer pair.

- The annealing temperature of a primer is strongly influenced by its nucleic acid sequence AND the reaction composition (pH, salts and salt concentration of the reaction buffer used). It has been shown that the annealing temperature with Genaxxon's LyoBalls is probably about 2-3°C lower compared to the original systems!