RealQ Plus 2x Master Mix Green - Without ROX
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Delivery time: 3-8 days
Product information "RealQ Plus 2x Master Mix Green - Without ROX"
Introduction:
Quantitative PCR is an important tool for SNP and gene expression analysis. Two general fluorescent chemistries exist for quantitative detection of gene transcripts: probes (e.g. TaqMan®, Scorpions™ Probes, molecular beacons) and DNA-binding fluorescent dyes (e.g. ethidium bromide, SYBR® Green, EvaGreen®, PicoGreen®). Ampliqon offers the RealQ Plus 2x Master Mix in two formulations: for probe or with DNA-binding fluorescent dye, making them ideal for most quantitative PCR applications.
The RealQ Plus 2x Master Mixes are available with high, low or without ROX™ for optimal performance on most of the commonly used real-time PCR instruments. The RealQ Plus 2x Master Mixes promote high specificity and low background by using TEMPase Hot Start DNA Polymerase, a modified Taq DNA polymerase with hot start capabilities.
The RealQ Plus 2x Master Mix Green, without ROX™, is a singletube 2x reagent including all components necessary to perform DNA-binding dye based real-time DNA amplification. Just add your primers and DNA.
Detection limit: approximately 1 copy.
Quantitation limit: approximately 24 copies (0.08ng of human gDNA, correlating to 12 diploid genomes, with 2 gene copies per diploid genome)
Compatibility: Real-time PCR instruments with no requirement for normalization to an internal reference dye.
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