GNX Versa qPCR MasterMix 2X
Advantages at a glance
- Built in Hot start PCR
- With special passive reference dye
- For all different PCR instruments
Product number:
M3212.0200
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.
Shipment: on wet ice. Store at -20°C. For laboratory usage only!
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.
Shipment: on wet ice. Store at -20°C. For laboratory usage only!
Product information "GNX Versa qPCR MasterMix 2X"
Make a better choice! No choosing of different qPCR master mixes anymore! One product for use with different thermal cycler simplifies the decision making process.
The GNX Versa qPCR Master Mix 2X is an optimized 2-time reaction mix for real-time qPCR detection and quantitation of target DNA sequences using the SYBR®/FAM channel of most real-time qPCR instruments. It contains our HotStart Taq DNA Polymerase together with a unique passive reference dye that is compatible across a variety of instrument platforms (including those that require a high or low ROX reference signal). This dye does not spectrally overlap with fluorescent dyes used for qPCR and will not interfere with real-time detection.
The master mix formulation contains all PCR components required for amplification and quantitation of DNA except primers and DNA template. Genomic DNA or cDNA can be analysed using existing as well as commercial primers.
Advantages
- One formulation for different plattforms
- Convenient master mix format
- User-friendly protocols simplifing reaction setup
- Product performs consistently across a wide variety of sample source
Data analysis
SYBR® Green binds non-specifically to double-stranded DNA. For this, the best way to check if a qPCR reaction using
SYBR® Green
amplified a single target without primer dimer formation is with melt-curve analysis. Melt-curve analysis is performed after a qPCR run and serves to verify that PCR amplification was specific for a single amplicon. The nonspecific binding of SYBR® Green makes melt-curve analysis one of the very few ways to confirm target-specific amplification.
If the fluorescence signal generated is due to primer dimers or non-specific amplification, your relative quantification of target is at least suspect. So when starting up a new SYBR® Green PCR, always perform melt-curve analysis as a QC step to help confirm target specificity!
Consistent performance when using different instruments and modes

Strong performance with multiple genes of various species

Robustness against thawing and freezing cycles

2-time qPCR master mix with special passive reference dye usable for all kind of PCR instruments.
Applikation / Application:
real-time qPCR, DNA and cDNA quantification
Einheiten / Units:
Quelle / Source:
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-05-02
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.
Documents:
Safety Data SheetManuals
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed
Source: NCBI PubMed
Data Analysis
1. Draw a standard curve according to Ct values of endogenous gene. The value of R2 should be more than 0.98 and the slope of curve should be in the range of -3 to -3.5 which means the PCR amplification efficiency is in the range of 90% to 120%.
2. The standard deviation (STD) of Ct values should be less than 0.2 and the variation of Ct value for different experiment should be less than 0.5 (the threshold value of different experiments should be same when comparing Ct values).
3. A single peak in the melt curve indicates, but does not prove, that there is no non-specific amplification product or primer dimmers. It can be confirmed by analyzing the PCR amplification product by agarose gel electrophoresis. Observing a single band on the gel is further evidence that a single product was amplified.
4. Observing multiple peaks, shoulders on the main peak, unusually wide peaks or asymmetrical peaks suggests that primer-dimers formed or that non-specific amplification occurred.
qPCR optimization in focus: tips and tricks for excellent analyses
December 12, 2023
Science
Real-time PCR (qPCR) is undoubtedly an impressive method for measuring the amplification of target DNA in real time.