Genaxx1Step RT-qPCR SARS-CoV-2 Probe Kit

The Genaxx1Step one-step RT-qPCR SARS-CoV-2 Probe Kit contains our innovative HotScriptase RT which is an extremely thermo- and inhibitor stable DNA polymerase with reverse transcriptase activity which does not need purified RNA for transcription and amplification. The eluate of a swab is enough for direct RT-PCR.  Our kit is a cheaper alternative to Diasorin kit with an ability to deliver similar results. Also unlike some of our competitor´s kits, Genaxx1step RT-PCR SARS-CoV-2 Probe Kit is less sensitive to inhibitors and can be used on commonly used real time PCR machines such as Lightcycler 480.
The HotScriptase polymerase facilitates an “OneStep” RT-PCRs directly from RNA templates, as reverse transcription takes place simultaneously with DNA amplification during the cycled PCR elongation step. Besides the very convenient protocol this allows reverse transcription reactions at higher temperatures, thus minimizing the problems encountered with strong secondary structures in RNA, or strong tertiary structures as in double strand RNA viruses that melt at elevated temperatures. High tempertures helps implementing a heat denaturation step sufficient to destroy the viral envelope and thus releasing the viral RNA. Furthermore, high temperatures help to destroy secondary structure structures in the viral RNA which are often found in single-stranded RNA viruses, e.g. pseudoknots, loops and hairpins, which represent a difficult target for other transcriptases than HotScriptase and can cause the enzyme to detach from the RNA strand. Efficient transcription is thus often not possible but in the case of our Covid-19 direct kit, a temperature of 75°C is applied during the transcription process, which eliminates such possible blocks.

The assay is based on the CDC-approved EUA SARS-CoV2 primer/probes sets. In accordance with the aforementioned publication, N1-assay was supplemented with a high-melting primer R2 to improve assay performance with Genaxx1Step RT-qPCR SARS-CoV-2 Probe Kit.

Intended Usage
This document describes the use of real-time RT-PCR assays for the in vitro qualitative detection of SARS-CoV-2 Corona virus nucleocapsid fragment target N1 and the control target RNASeP according to the US Centers for Disease Control and Prevention (CDC), which published a protocol for the detection of the 2019-Novel Coronavirus (2019-nCoV). The 2019-nCoV primer and probe sets are designed for the specific detection of 2019-nCoV.

Genaxx1Step RT-qPCR SARS-CoV-2 Probe Assay Kit Detection system
Genaxx1Step RT-qPCR SARS-CoV-2 Probe Kit is a real-time RT-PCR based detection system for the 2019 Wuhan coronavirus (2019-nCoV). 2019-nCoV is considered a novel human coronavirus that is genetically distinct from the common human coronaviruses (229E, NL63, OC43, HKU1), which cause seasonal acute respiratory illness. It is also genetically distinct from the two newer human coronaviruses, MERS-CoV and SARS-CoV.

Genaxx1Step RT-qPCR SARS-CoV-2 Probe Kit detects the presence of the nucleocapsid fragment sequences N1 of 2019-nCoV / SARS-CoV-2 and the control target RNASeP even without prior RNA extraction.

NOTE: Always include positive and negative controls in your experiment to validate your results.